桒原 正靖
| 文理学部 化学科 | 教授 |
研究者情報
プロモーション
プロフィール
バイオ分析化学, バイオ医薬品化学, 生物有機化学および進化分子工学等を基盤としてバイオ分析・医学関連分野の研究開発を行っています。 研究室URL: http://kuwaharaken.org/
経歴
- 2018年04月 - 現在
学校法人 日本大学, 文理学部 化学科, 教授 - 2013年04月 - 2018年03月
国立大学法人 群馬大学, 大学院理工学府, 准教授 - 2009年12月 - 2013年03月
国立大学法人 群馬大学, 大学院工学研究科, 准教授 - 2007年04月 - 2009年11月
国立大学法人 群馬大学, 大学院工学研究科, 助教 - 2004年10月 - 2008年03月
独立行政法人 科学技術振興機構, 戦略的創造研究推進事業（さきがけ）「構造機能と計測分析」領域（寺部 茂 研究総括）, さきがけ研究者（兼任） - 2004年04月 - 2007年03月
国立大学法人 群馬大学, 工学部 応用化学科, 助手 - 2001年06月 - 2004年03月
国立 群馬大学, 工学部 応用化学科, 助手 - 2000年04月 - 2001年05月
米国バージニア大学, 化学科, 博士研究員 - 1999年04月 - 2000年03月
国立 岡山大学, 工学部, 非常勤講師
研究活動情報
論文
- ★DNA polymerase variants with high processivity and accuracy for encoding and decoding locked nucleic acid sequences
Hidekazu Hoshino; Yuuya Kasahara; Masayasu Kuwahara; Satoshi Obika
Journal of the American Chemical Society, 2020年12月
責任著者 - ★Seconds-resolved pharmacokinetic measurements of the chemotherapeutic irinotecan: In situ in the living body
Andrea Idili; Netzahualcóyotl Arroyo-Currás; Kyle L. Ploense; Andrew T. Csordas; Masayasu Kuwahara; Tod E. Kippin; Kevin W. Plaxco
Chemical Science, 2019年 - ★In situ condensation of an anti-cancer drug into fibrin gel enabling effective inhibition of tumor cell growth
Masayasu Kuwahara; Hiroto Fujita; Yuka Kataoka; Yasuyo Nakajima; Masanobu Yamada; Naoki Sugimoto
Chemical Communications, 2019年
筆頭著者 - ★Novel one-tube-one-step real-time methodology for rapid transcriptomic biomarker detection: Signal amplification by ternary initiation complexes
Hiroto Fujita; Yuka Kataoka; Seiji Tobita; Masayasu Kuwahara; Naoki Sugimoto
Analytical Chemistry, 2016年07月
責任著者 - ★Minimal thioflavin T modifications improve visual discrimination of guanine-quadruplex topologies and alter compound-induced topological structures
Yuka Kataoka; Hiroto Fujita; Yuuya Kasahara; Toshitada Yoshihara; Seiji Tobita; Masayasu Kuwahara
Analytical Chemistry, 2014年12月
責任著者 - ★Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine tRNAs
Debabrata Mandal; Caroline Köhrer; Dan Su; I. Ramesh Babu; Clement T.Y. Chan; Yuchen Liu; Dieter Söll; Paul Blum; Masayasu Kuwahara; Peter C. Dedon; Uttam L. Rajbhandary
RNA, 2014年02月 - ★Efficacy of base-modification on target binding of small molecule DNA aptamers
Yuri Imaizumi; Yuuya Kasahara; Hiroto Fujita; Shunsuke Kitadume; Hiroaki Ozaki; Tamaki Endoh; Masayasu Kuwahara; Naoki Sugimoto
Journal of the American Chemical Society, 2013年06月
責任著者 - ★Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection of base- and sugar-modified DNA aptamers: Target binding dominated by 2'- O,4'-C-methylene-bridged/locked nucleic acid primer
Yuuya Kasahara; Yuuta Irisawa; Hiroto Fujita; Aiko Yahara; Hiroaki Ozaki; Satoshi Obika; Masayasu Kuwahara
Analytical Chemistry, 2013年05月
責任著者 - ★Artificially created nucleic acids and peptides/proteins in chemical biology
Masayasu Kuwahara; Yingfu Li; Eriks Rozners; Hiroshi Murakami
Journal of Nucleic Acids, 2013年
筆頭著者 - ★Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides
Masayasu Kuwahara; Satoshi Obika; Jun Ichi Nagashima; Yuki Ohta; Yoshiyuki Suto; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi
Nucleic Acids Research, 2008年08月
筆頭著者 - ★Modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity
Atsushi Shoji; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Journal of the American Chemical Society, 2007年02月 - ★Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides
Shu Ichi Nakano; Toshimasa Kirihata; Satoshi Fujii; Hiroshi Sakai; Masayasu Kuwahara; Hiroaki Sawai; Naoki Sugimoto
Nucleic Acids Research, 2007年01月 - ★Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA
Masayasu Kuwahara; Jun Ichi Nagashima; Masatoshi Hasegawa; Takehiro Tamura; Rina Kitagata; Kazuo Hanawa; Shin Ichi Hososhima; Toshiyuki Kasamatsu; Hiroaki Ozaki; Hiroaki Sawai
Nucleic Acids Research, 2006年11月
筆頭著者 - ★Nucleic acid with guanidinium modification exhibits efficient cellular uptake
Tatsuo Ohmichi; Masayasu Kuwahara; Naoko Sasaki; Masatoshi Hasegawa; Takahito Nishikata; Hiroaki Sawai; Naoki Sugimoto
Angewandte Chemie - International Edition, 2005年10月 - ★Design and optimization of camptothecin conjugates of triple helix-forming oligonucleotides for sequence-specific DNA cleavage by topoisomerase I
Paola B. Arimondo; Alexandre Boutorine; Brigitte Baldeyrou; Christian Bailly; Masayasu Kuwahara; Sidney M. Hecht; Jian Sheng Sun; Thérèse Garestier; Claude Hélène
Journal of Biological Chemistry, 2002年02月 - ★Hybridization between oxy-peptide nucleic acids and DNAs: Dependence of hybrid stabilities on the chain-lengths, types of base pairs, and the chain directions
Masayasu Kuwahara, Miki Arimitsu, Masanori Shigeyasu, Naho Saeki, Masahiko Sisido
Journal of the American Chemical Society, 2001年
筆頭著者 - ★Novel peptide nucleic acid that shows high sequence specificity and all- or-none-type hybridization with the complementary DNA
Masayasu Kuwahara; Miki Arimitsu; Masahiko Sisido
Journal of the American Chemical Society, 1999年01月
筆頭著者 - Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review
Yilei Wang, Mohan Amarasiri, Wakana Oishi, Masayasu Kuwahara, Yuka Kataoka, Hiroki Kurita, Fumio Narita, Rong Chen, Qian Li, Daisuke Sano
Water Research, 2025年03月 - ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead
Masumi Taki; Masayasu Kuwahara; Chaohui Li; Naoko Tomoda; Naoyuki Miyashita; Tetsuo Kan; Jay Yang
Chemical Communications, 2024年12月 - Lantern-type G-quadruplex fluorescent sensors for detecting divalent metal ions
Tomoko Wariishi; Yuka Kataoka; Tomoaki Nakamura; Yuuya Kasahara; Masataka Kuroda; Satoshi Obika; Masayasu Kuwahara
Analytical Biochemistry, 2024年07月 - Field-effect transistor biosensor with signal amplification by ternary initiation complexes for detection of wide-range RNA concentration.
Hiroki Hayashi; Akihiro Enami; Hiroto Fujita; Shigeki Kuroiwa; Keishi Ohashi; Masayasu Kuwahara; Tetsuya Osaka; Toshiyuki Momma
Talanta, 2024年06月 - Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method
Kunihiko Morihiro; Osamu Hasegawa; Yuuya Kasahara; Shohei Mori; Tatsuro Kasai; Masayasu Kuwahara; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, 2021年01月 - Bifunctional aptamer drug carrier enabling selective and efficient incorporation of an approved anticancer drug irinotecan to fibrin gels
Hiroto Fujita; Yuka Kataoka; Masayasu Kuwahara
Applied Sciences (Switzerland), 2020年12月 - Effects of Modifying Thioflavin T at the N3-Position on Its G4 Binding and Fluorescence Emission
Yuka Kataoka; Hiroto Fujita; Tamaki Endoh; Naoki Sugimoto; Masayasu Kuwahara
Molecules (Basel, Switzerland), 2020年10月 - A high affinity modified DNA aptamer containing base-appended bases for human β-defensin
Hirotaka Minagawa; Yuka Kataoka; Masayasu Kuwahara; Katsunori Horii; Ikuo Shiratori; Iwao Waga
Analytical Biochemistry, 2020年04月 - Modified DNA aptamers for C-reactive protein and lactate dehydrogenase-5 with sub-nanomolar affinities
Hirotaka Minagawa; Yuka Kataoka; Hiroto Fujita; Masayasu Kuwahara; Katsunori Horii; Ikuo Shiratori; Iwao Waga
International Journal of Molecular Sciences, 2020年04月 - Fluorescence Polarization-Based Rapid Detection System for Salivary Biomarkers Using Modified DNA Aptamers Containing Base-Appended Bases
Hirotaka Minagawa; Akihisa Shimizu; Yuka Kataoka; Masayasu Kuwahara; Shintaro Kato; Katsunori Horii; Ikuo Shiratori; Iwao Waga
Analytical Chemistry, 2020年01月 - High-Contrast Facile Imaging with Target-Directing Fluorescent Molecular Rotors, the N3-Modified Thioflavin T Derivatives
Yuka Kataoka; Hiroto Fujita; Arina Afanaseva; Chioko Nagao; Kenji Mizuguchi; Yuuya Kasahara; Satoshi Obika; Masayasu Kuwahara
Biochemistry, 2019年02月 - Selective incorporation of foreign functionality into fibrin gels through a chemically modified DNA aptamer
Hiroto Fujita; Yusuke Inoue; Masayasu Kuwahara
Bioorganic and Medicinal Chemistry Letters, 2018年01月 - Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1
Keisuke Tanaka; Yuuya Kasahara; Yoichi Miyamoto; Okuda Takumi; Tatsuro Kasai; Kentaro Onodera; Masayasu Kuwahara; Masahiro Oka; Yoshihiro Yoneda; Satoshi Obika
Organic and Biomolecular Chemistry, 2018年 - Synthesis and properties of microenvironment-sensitive oligonucleotides containing a small fluorophore, 3-aminobenzonitrile or 3-aminobenzoic acid
Hiroaki Ozaki; Takeshi Kawai; Masayasu Kuwahara
Tetrahedron, 2017年12月 - Specific light-up system for protein and metabolite targets triggered by initiation complex formation
Hiroto Fujita; Yuka Kataoka; Remi Nagano; Yasuyo Nakajima; Masanobu Yamada; Naoki Sugimoto; Masayasu Kuwahara
Scientific Reports, 2017年12月 - Selection, Characterization and Application of Artificial DNA Aptamer Containing Appended Bases with Sub-nanomolar Affinity for a Salivary Biomarker
Hirotaka Minagawa; Kentaro Onodera; Hiroto Fujita; Taiichi Sakamoto; Joe Akitomi; Naoto Kaneko; Ikuo Shiratori; Masayasu Kuwahara; Katsunori Horii; Iwao Waga
Scientific Reports, 2017年03月 - Real-Time Monitoring of G-Quadruplex Formation during Transcription
Tamaki Endoh; Ambadas B. Rode; Shuntaro Takahashi; Yuka Kataoka; Masayasu Kuwahara; Naoki Sugimoto
Analytical Chemistry, 2016年02月 - Consecutive incorporation of functionalized nucleotides with amphiphilic side chains by novel KOD polymerase mutant
Hidekazu Hoshino; Yuuya Kasahara; Hiroto Fujita; Masayasu Kuwahara; Kunihiko Morihiro; Shin Ichi Tsunoda; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, 2016年01月 - Non-Equilibrium Capillary Electrophoresis of Equilibrium Mixtures-Based Affinity Separation and Selective Enrichment of a Long-Length DNA Aptamer
Kenta Hagiwara; Yuuya Kasahara; Hiroto Fujita; Masayasu Kuwahara
Australian Journal of Chemistry, 2016年 - Selection of natural and base-modified DNA aptamers for a camptothecin derivative
Hiroto Fujita; Masayasu Kuwahara
Current Protocols in Nucleic Acid Chemistry, 2016年 - Polymerase incorporation of a 2'-deoxynucleoside-5'-triphosphate bearing a 4-hydroxy-2-mercaptobenzimidazole nucleobase analogue
Kunihiko Morihiro; Hidekazu Hoshino; Osamu Hasegawa; Yuuya Kasahara; Kohsuke Nakajima; Masayasu Kuwahara; Shin Ichi Tsunoda; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, 2015年06月 - Polymerase-mediated high-density incorporation of amphiphilic functionalities into DNA: Enhancement of nuclease resistance and stability in human serum
Hiroto Fujita; Kohsuke Nakajima; Yuuya Kasahara; Hiroaki Ozaki; Masayasu Kuwahara
Bioorganic and Medicinal Chemistry Letters, 2015年01月 - In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids
Kenta Hagiwara; Hiroto Fujita; Yuuya Kasahara; Yuuta Irisawa; Satoshi Obika; Masayasu Kuwahara
Molecular BioSystems, 2015年01月 - Creation of nucleic acid aptamers that contain unnatural nucleotides
Masayasu Kuwahara
Kobunshi, 2014年10月 - 2',4'-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides
Yuuya Kasahara; Yuuta Irisawa; Hiroaki Ozaki; Satoshi Obika; Masayasu Kuwahara
Bioorganic and Medicinal Chemistry Letters, 2013年03月 - Cleavage of supercoiled plasmid DNA by phenanthrolinepolyamine conjugates as a metal-free artificial nuclease
Tomokazu Masuda; Takeshi Sasagase; Akiko N. Ozaki; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Chemistry Letters, 2013年01月 - Structural and affinity analyses of G-quadruplex DNA aptamers for camptothecin derivatives
Hiroto Fujita; Yuri Imaizumi; Yuuya Kasahara; Shunsuke Kitadume; Hiroaki Ozaki; Masayasu Kuwahara; Naoki Sugimoto
Pharmaceuticals, 2013年 - In vitro selection of BNA (LNA) aptamers
Masayasu Kuwahara; Satoshi Obika
Artificial DNA: PNA and XNA, 2013年 - Specific amino acid sensing using a single acridone-labeled DNA aptamer
Hiroaki Ozaki; Yasuhisa Hagiwara; Masayasu Kuwahara
Chemistry Letters, 2012年 - Artificial specific binders directly recovered from chemically modified nucleic acid libraries
Yuuya Kasahara; Masayasu Kuwahara
Journal of Nucleic Acids, 2012年 - Optimization of pyrosequencing reads by superior successive incorporation efficiency of improved 2'-deoxyadenosine-5'-triphosphate analogs
Tomoharu Kajiyama; Masayasu Kuwahara; Mari Goto; Hideki Kambara
Analytical Biochemistry, 2011年09月 - Synthesis of modified siRNA bearing C-5 polyamine-substituted pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity
Mohammad Mehedi Masud; Tomokazu Masuda; Yusuke Inoue; Masayasu Kuwahara; Hiroaki Sawai; Hiroaki Ozaki
Bioorganic and Medicinal Chemistry Letters, 2011年01月 - Nucleotide modification and polymerase engineering for creating a novel class of artificial nucleic acid aptamers
Y. Imaizumi; Y. Takano; S. Kitadume; H. Ozaki; S. Obika; M. Kuwahara
IFMBE Proceedings, 2011年 - Study on suitability of kod dna polymerase for enzymatic production of artificial nucleic acids using base/sugar modified nucleoside triphosphates
Masayasu Kuwahara; Yuuki Takano; Yuuya Kasahara; Hiroki Nara; Hiroaki Ozaki; Hiroaki Sawai; Akio Sugiyama; Satoshi Obika
Molecules, 2010年11月 - Molecular evolution of functional nucleic acids with chemical modifications
Masayasu Kuwahara; Naoki Sugimoto
Molecules, 2010年08月 - Effect of 3'-end capping of aptamer with various 2',4'-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers
Yuuya Kasahara; Shunsuke Kitadume; Kunihiko Morihiro; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, 2010年03月 - Polymerase reactions using artificial nucleic acids and creation of artificial nucleic acid aptamers
Masayasu Kuwahara
Seikagaku, 2010年 - Synthesis and properties of oligodeoxyribonucleotides bearing a polyamino group at the 2' position via 2'-O-carbamoylmethyl and 2'-S-carbamoylmethyl groups
Hiroaki Ozaki; Shingo Nomura; Shingo Momiyama; Kazuyuki Yokotsuka; Masayasu Kuwahara; Hiroaki Sawai
Nucleosides, Nucleotides and Nucleic Acids, 2009年10月 - Smart conferring of nuclease resistance to DNA by 3'-end protection using 2',4'-bridged nucleoside-5'-triphosphates
Masayasu Kuwahara; Satoshi Obika; Hidetoshi Takeshima; Yoshihiko Hagiwara; Jun ichi Nagashima; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi
Bioorganic and Medicinal Chemistry Letters, 2009年06月 - Fluorescent properties of acridonyl group in DNA duplex.
Masayasu Kuwahara; Atsushi Kobayashi; Masayoshi Tajima; Shunsuke Kitazume; Keisuke Anahara; Hiroaki Ozaki; Seiji Tobita
Nucleic acids symposium series (2004), 2009年 - Acridone-labeled DNA aptamer for the detection of biomolecules.
Hiroaki Ozaki; Yasuhisa Hagiwara; Hiromi Asakura; Masayasu Kuwahara
Nucleic acids symposium series (2004), 2009年 - Acridone-tagged DNA as a new probe for DNA detection by fluorescence resonance energy transfer and for mismatch DNA recognition
Yasuhisa Hagiwara; Tomoya Hasegawa; Atushi Shoji; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry, 2008年07月 - Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosolute as a mimic of the intracellular media
Shu-ichi Nakano; Lei Wu; Hirohito Oka; Hisae Tateishi Karimata; Toshimasa Kirihata; Yuichi Sato; Satoshi Fujii; Hiroshi Sakai; Masayuki Kuwahara; Hiroaki Sawai; Naoki Sugimoto
Molecular BioSystems, 2008年 - Arginine-modified DNA aptamers that show enantioselective recognition of the dicarboxylic acid moiety of glutamic acid
Kazuomi Ohsawa; Toshiyuki Kasamatsu; Jun Ichi Nagashima; Kazuo Hanawa; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Analytical Sciences, 2008年01月 - Effect of backbone-modification of oligodeoxyribonucleic acid on primer extension reactions.
Masayasu Kuwahara; Satoshi Minezaki; Jun ichi Nagashima; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2008年 - Synthesis and incorporation of fluorescent C-nucleoside analogue into oligodeoxyribonucleotides.
Hiroaki Ozaki; Takeshi Kawai; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2008年 - Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative
Atsushi Shoji; Tomoya Hasegawa; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, 2007年02月 - Polymerisation of a DNA strand using oligo-DNA template with modified bases, sugars and phosphates.
Jun Ichi Nagashima; Satoshi Minezaki; Satoshi Obika; Takeshi Imanishi; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2007年 - Synthesis of novel fluorescent nucleoside bearing 3-aminobenzonitrile as a nucleobase.
Hiroaki Ozaki; Takahiro Setaka; Syouta Tsuchiya; Takeshi Kawai; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2007年 - Interaction with and cleavage of DNA by novel phenanthroline polyamine derivative.
Takeshi Sasagase; Takeshi Arai; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2007年 - Differences in substrate specificity of C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides by DNA polymerases from thermophilic bacteria, archaea, and phages
Hiroaki Sawai; Junichi Nagashima; Msayasu Kuwahara; Rina Kitagata; Takehiro Tamura; Ikuo Matsui
Chemistry and Biodiversity, 2007年 - Biomolecular sensor based on fluorescence-labeled aptamer
Hiroaki Ozaki; Akifumi Nishihira; Masayuki Wakabayashi; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, 2006年08月 - Direct PCR amplification of various modified DNAs having amino acids: Convenient preparation of DNA libraries with high-potential activities for in vitro selection
Masayasu Kuwahara; Kazuo Hanawa; Kazuomi Ohsawa; Rina Kitagata; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry, 2006年04月 - Substrate property and incorporation accuracy of various dATP analogs during enzymatic polymerization using thermostable DNA polymerases.
Masayasu Kuwahara; Yoshiyuki Suto; Satoshi Minezaki; Rina Kitagata; Jun ichi Nagashima; Hiroaki Sawai
Nucleic acids symposium series (2004), 2006年 - Synthesis and property of DNA labeled with fluorescent acridone.
Tomoya Hasegawa; Atsusi Shoji; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2006年 - Assembly of DNA nanostructures with branched tris-DNA
Takahiro Kuroda; Yoshimasa Sakurai; Yukinao Suzuki; Akiko O. Nakamura; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Chemistry - An Asian Journal, 2006年 - Expansion of repertoire of modified DNAs prepared by PCR using KOD Dash DNA polymerase
Tsutomu Ohbayashi; Masayasu Kuwahara; Masatoshi Hasegawa; Toshiyuki Kasamatsu; Takehiro Tamura; Hiroaki Sawai
Organic and Biomolecular Chemistry, 2005年07月 - Screening of a glutamic acid-binding aptamer from arginine-modified DNA library.
Masayasu Kuwahara; Kazuomi Ohsawa; Toshiyuki Kasamatsu; Atsushi Shoji; Hiroaki Sawai; Hiroaki Ozaki
Nucleic acids symposium series (2004), 2005年 - Coformational switch of oligonucleotide induced by spermine.
Toshimasa Kirihata; Shu ichi Nakano; Satoshi Fujii; Manabu Matsumoto; Masayasu Kuwahara; Hiroaki Sawai; Naoki Sugimoto
Nucleic acids symposium series (2004), 2005年 - Comparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates.
Masayasu Kuwahara; Takehiro Tamura; Rina Kitagata; Hiroaki Sawai; Hiroaki Ozaki
Nucleic acids symposium series (2004), 2005年 - Synthesis and properties of oligonucleotides containing 5-substituted pyrimidine nucleoside
Hiroaki Ozaki; Masayasu Kuwahara; Hiroaki Sawai
Yuki Gosei Kagaku Kyokaishi/Journal of Synthetic Organic Chemistry, 2004年12月 - Synthesis and antisense properties of oligodeoxyribonucleotides containing C5-substituted arabinofuranosyluracil
Hiroaki Ozaki; Kiyohiro Nakajima; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, 2004年03月 - Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX
Mohammad Mehedi Masud; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry, 2004年03月 - Detection of biomolecule by aptamer beacon.
Akifumi Nishihira; Hiroaki Ozaki; Masayuki Wakabayashi; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2004年 - Screening of modified DNA aptamers that recognize DNA secondary structure.
Masayasu Kuwahara; Toshiyuki Kasamatsu; Masatoshi Hasegawa; Atsushi Shoji; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2004年 - Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA polymerases during PCR
Masayasu Kuwahara; Yumi Takahata; Atsushi Shoji; Akiko N. Ozaki; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, 2003年11月 - Convenient synthesis of oligodeoxyribonucleotides bearing arabinofuranosyl pyrimidine derivatives and its duplex formation with complementary DNA
Hiroaki Ozaki; Kiyohiro Nakajima; Kaoru Tatsui; Chieko Izumi; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, 2003年08月 - Modified DNA bearing 5-(methoxycarbonyl-methyl)-2′-deoxyuridine: Preparation by PCR with thermophilic DNA polymerase and post-synthetic derivatization
Mohammad Mehedi Masud; Akiko Ozaki-Nakamura; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
ChemBioChem, 2003年07月 - Simultaneous incorporation of three different modified nucleotides during PCR.
Masayasu Kuwahara; Shin ichi Hososhima; Yumi Takahata; Rina Kitagata; Atsushi Shoji; Kazuo Hanawa; Akiko N. Ozaki; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2003年 - Synthesis and properties of oligonucleotides containing C-2 and/or C-5 substituted arabinofuranosyluracils.
Hiroaki Ozaki; Kiyohiro Nakajima; Kyoko Yajima; Kaoru Tatsui; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2003年 - Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase
Tsutomu Obayashi; Mohammed M. Masud; Akiko N. Ozaki; Hiroaki Ozaki; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, 2002年04月 - Enzymatic incorporation of chemically-modified nucleotides into DNAs.
Masayasu Kuwahara; Tsutomu Ohbayashi; Kazuo Hanawa; Atsushi Shoji; Akiko N. Ozaki; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2002年 - Modified DNA aptamers against sweet agent aspartame.
Hiroshi Saitoh; Akiko Nakamura; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2002年 - Synthesis and DNA binding property of a novel peptide nucleic acid that contains cis-4-adeninyl-L-prolinol unit
Masanori Shigeyasu; Masayasu Kuwahara; Masahiko Sisido; Teruhiko Ishikawa
Chemistry Letters, 2001年 - Synthesis of δ-amino acids with an ether linkage in the main chain and nucleobases on the side chain as monomer units for oxy-peptide nucleic acids
Masayasu Kuwahara; Miki Arimitsu; Masahiko Sisido
Tetrahedron, 1999年08月 - Sequence-specific hybridization between two different types of peptide nucleic acids
Masayasu Kuwahara; Miki Arimitsu; Masahiko Sisido
Bulletin of the Chemical Society of Japan, 1999年07月 - Synthesis of oxy-peptide nucleic acids with mixed sequences.
M. Kuwahara; M. Arimitsu; M. Sigeyasu; M. Sisido
Nucleic acids symposium series, 1999年 - Peptide Nucleic Acid with Ether Linkages in the Main Chain. Synthesis and Sequence-Specific Interaction with DNA
Masahiko Sisido, Miki Arimitsu, Masayasu Kuwahara
1998年10月
書籍等出版物
- ★Handbook of chemical biology of nucleic acids (edited by Naoki Sugimoto)
Fujita H; Kuwahara M, 分担執筆, Detection systems using the ternary complex formation of nucleic acids
Springer, Singapore, 2022年10月
9789811613135 - ★Modified nucleic acids in biology and medicine (RNA technologies) (edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski)
Kuwahara M; Hagiwara K; Ozaki H, 分担執筆, Polymerase reactions that involve modified nucleotides
Springer, Heidelberg, 2016年08月
9783319341736 - ★Chemical biology of nucleic acids: Fundamentals and clinical applications (RNA technologies) (edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski)
Kuwahara M, 分担執筆, Progress in chemically modified nucleic acid aptamers
Springer, Heidelberg, 2014年05月
9783642544514 - ★DNA sequencing–methods and apprications (edited by Anjana Munshi)
Kuwahara M; Sugimoto N, 分担執筆, Nucleic acid aptamers as molecular tags for omics analyses involving sequencing
INTECH-Open Access Publisher, Croatia, 2012年04月
9535105647 - CSJカレントレビュー：進化を続ける核酸化学（日本化学会 編）
片岡由佳; 桒原正靖, 分担執筆, PartⅡ 研究最前線（5章）化学修飾を導入した核酸アプタマー開発の進展
化学同人, 2021年10月
9784759814019 - 核酸科学ハンドブック（日本核酸化学会 監，杉本直己 編）
桒原正靖, 分担執筆, 第Ⅱ部 核酸科学の最前線（23）核酸鎖関連バイオマーカーの簡易検出法
講談社, 2020年12月
9784065207864 - CSJカレントレビュー：生体分子反応を制御する（日本化学会 編）
桒原正靖; 杉本直己, 分担執筆, 第Ⅱ部 研究最前線（11章）相分離や分子クラウディングによる核酸の機能制御
化学同人, 2020年04月
9784759813968 - 中分子創薬に資するペプチド・核酸・糖鎖の合成技術（千葉一裕 監修）
萩原健太; 尾崎広明; 桒原正靖, 分担執筆, ゼノ核酸アプタマーの開発
シーエムシー出版, 2018年02月
9784781313207 - CSJカレントレビュー：核酸化学のニュートレンド（日本化学会 編）
桒原正靖, 分担執筆, PartⅡ 研究最前線（5章）核酸アプタマー
化学同人, 2011年07月
9784759813661 - シングルセル解析の最前線（神原秀記，松永是，植田充美 編）
桒原正靖; 梶山智晴, 分担執筆, （4.5）修飾基質アナログを用いたポリメラーゼ反応系と遺伝子配列解析法
シーエムシー出版, 2010年03月
9784781302034 - Frontiers in organic chemistry. Volume 1 (edited by Atta-ur-Rahman, Yoshihiro Hayakawa)
Ozaki H; Kuwahara M; Sawai H, 分担執筆, Oligonucleotides bearing α5-substituted pyrimidine nucleoside: Their synthesis, properties, and application
Bentham Science Publishers Ltd., Sharjah., 2005年03月
9077527060 - ゲノムケミストリー（関根光雄，斎藤烈 編）
澤井宏明; 桒原正靖, 分担執筆, （4.2）人工修飾核酸を用いた試験管内選択法（セレックス法）の拡張
講談社サイエンティフィック, 2003年03月
9784061543157 - Self-assembling peptide systems in biology, medicine and engineering (edited by Amalia Aggeli, Neville Boden, Shuguang Zhang)
Sisido M; Kuwahara M, 分担執筆, Novel peptide nucleic acids with improved solubility and DNA-binding ability
Kluwer Academic Publishers, Boston., 2001年12月
9780792370901
講演・口頭発表等
- ★Enhanced selectivity over topology and altered targeting by fluorescent G4 binder modifications
Kuwahara M.
Asian 3 Roundtable on Nucleic Acids 2024 (A3RONA 2024), Nov 2, 2024, 2024年11月, 招待有り - ★Fluorescent binders with enhanced selectivity for G-quadruplex topologies
Kuwahara M
Advances in Noncanonical Nucleic Acids (ANNA 2024), Oct 26, 2024, 2024年10月, 招待有り - ★Halfway through the development of technology to create molecular recognition polymers that function in water
Kuwahara M
Supra FIBER International Summit for Nucleic Acids (S-FISNA), 2024年02月, 招待有り - ★Modified substrates and polymerase variants for functional xeno nucleic acid selections
Kuwahara M
FIBER International Summit for Nucleic Acids 2023 (FISNA2023), 2023年07月, 招待有り - ★Fluorescence molecular rotors Thioflavin T derivatives for various targets
Kuwahara M
The 2nd International Symposium on Functional Nucleic Acids (FNA2018), 2018年11月, 招待有り - ★Development of unique nucleic acid materials for diagnosis and therapeutics
Kuwahara M
NanoTech Poland 2018, 2018年06月, 招待有り - ★Xenonucleic acid aptamers: development towards in vitro selection of artificial biopolymer ligands/receptors
Kuwahara M
The 32nd International Symposium on Microscale Separations and Bioanalysis (MSB 2016), 2016年04月, 招待有り - ★Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection using modified nucleic acid libraries
Kuwahara M
2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015), 2015年12月, 招待有り - ★Functional nucleic acids with chemical modifications
Kuwahara M
POLYCHEM Seminar (Host: Prof. D.A.Schluter, Swiss Federal Institute of Technology Zurich (ETHZ)), 2011年09月, 招待有り - Modified nucleic acid aptamer selections using polymerase variants and substrate analogs
Kuwahara M
Asian 3 Roundtable on Nucleic Acids 2023 (A3RONA 2023), Nov 11, 2023, 2023年11月, 招待有り - Detection systems that start with the ternary initiation complex formation
Kuwahara M
FIBER International Summit for Nucleic Acids 2022 (FISNA2022), 2022年07月, 招待有り - Xeno-nucleic acid aptamers: Progress in nucleic acid aptamer development with chemical modification
Kuwahara M
The 2nd Aptamer Seminar in Tokyo, 2021年07月, 招待有り - Thioflavin T modification and the application to the detection of biomarkers and viruses
Kuwahara M
13th JKBT: Asian Workshop against Coronavirus Disease, 2021年01月, 招待有り - Ultra-high sensitive simple test methods for various biomarkers
Kuwahara M
Advances in Noncanonical Nucleic Acids 2019 (ANNA2019), 2019年10月, 招待有り - Method for high sensitive detection of various biomarkers
Kuwahara M
Asian 3 Roundtable on Nucleic Acids 2019 (A3RONA 2019), 2019年10月, 招待有り - Simple test methods for various biomarkers at attomolar level
Kuwahara M
Poland-Japan RNA Meeting, Poznań 2019, 2019年06月, 招待有り - Target-directing fluorescent molecular rotors
Kuwahara M
Asian 3 Roundtable on Nucleic Acids 2018 (A3RONA 2018), 2018年09月, 招待有り - Simple method for various biomarker detections
Kuwahara M
Advances in Noncanonical Nucleic Acids (ANNA 2017), 2017年10月, 招待有り - One-tube-one-step real-time quantitative system for various biomolecular targets
Kuwahara M
Asia 3 Roundtable on Nucleic Acids 2017 (A3RONA 2017), 2017年09月, 招待有り - Specific light-up system for various biomarker detection
Kuwahara M
FIBER International Summit for Nucleic Acids 2017 (FISNA 2017), 2017年07月, 招待有り - Simple method for biomarker detection: Signal amplification by ternary initiation complexes
Kuwahara M
XVIIth Symposium on Chemistry of Nucleic Acid Components, 2017年06月, 招待有り - Signal amplification by ternary initiation complexes (SATIC) method for nucleic acid biomarker analyses
Kuwahara M
Japan-Spain Joint Workshop on Nanomedicine Research (AMED - MINECO – CSIC - ISCIII), 2016年12月, 招待有り - Application of unnatural nucleic acids and non-canonical nucleic acid structures towards drug discovery and bioanalysis
Kuwahara M
2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015), 2015年12月, 招待有り - Xeno-nucleic acid aptamers
Kuwahara M
The 1st International Symposium on “Functional Nucleic Acids: From Laboratory to Targeted Molecular Therapy” (FNA2015), 2015年11月, 招待有り - Syntheses and properties of novel Thioflavin T analogs
Kuwahara M
Asia 3 Roundtable on Nucleic Acids 2015 (A3RONA 2015), 2015年09月, 招待有り
所属学協会
産業財産権
- [Registered, Jan. 15, 2025], EP3957733（ドイツ，イギリス，フランス）
- [Registered, October 3, 2024], 特許第7565572号（日本）
- [Registered, Aug. 30, 2024], ZL202210227991.1（中国）
- [Registered, Mar. 4, 2024], 特許第7448239号（日本）
- [Registered, May 9, 2023], US11643660（米国）
- [Registered, Nov. 30, 2022], EP3546471（ドイツ，イギリス，フランス）
- [Registered, Nov. 1, 2022], 特許第7168920号（日本）
- [Registered, Sep. 27, 2022], ZL201780072997.3（中国）
- [Registered, Aug. 29, 2022], 特許第7131830号（日本）
- [Registered, Jun. 14. 2022], US11359240（米国）
- [Registered, May 17, 2022], ZL201780056678.3（中国）
- [Registered, May 4, 2022], EP3597776（ドイツ，フランス）
- [Registered, Mar. 22, 2022], ZL201780056368.1（中国）
- [Registered, Mar. 22, 2022], ZL201780056298.X（中国）
- [Registered, Feb. 1, 2022], US11236342（米国）
- [Registered, Jan. 31, 2022], 特許第7017246号（日本）
- [Registered, Jan. 21, 2022], 特許第7012957号（日本）
- [Registered, Jan. 18, 2022], 特許第7011145号（日本）
- [Registered, Dec. 27, 2021], 特許第6999976号（日本）
- [Registered, Nov. 3, 2021], EP3783105 (ドイツ，イギリス，フランス), DE602017048961.1
- [Registered, Oct. 19. 2021], 特許第6963221号（日本）
- [Registered, Jul. 14, 2021], EP3514237 (ドイツ，イギリス，フランス), DE602017042271.1
- [Registered, May 31, 2021], 特許第6892071号（日本）
- [Registered, May 19, 2021], EP3514165（ドイツ，イギリス，フランス）
- [Registered, Jan. 19, 2021], 特許第6826275号（日本）
- [Registered, Nov. 16, 2020], 特許第6795211号（日本）
- [Registered, Sep. 22, 2020], US10781230（米国）
- [Registered, Sep. 14, 2020], 特許第6763551号（日本）
- [Registered, Sep. 14, 2020], 特許第6763552号（日本）
- [Registered, Sep. 20, 2020], 特許第6763553号（日本）
- [Registered, Sep. 9, 2020], EP3514164（ドイツ，イギリス，フランス）
- [Registered, Sep. 1, 2020], US10760084（米国）
- [Registered, Aug. 3, 2020], 特許第6744028号（日本）
- [Registered, Apr. 7, 2020], US10611791（米国）
- [Registered, Mar. 34, 2020], US10597719（米国）
- [Registered, Aug. 20, 2019], US10385090（米国）
- [Registered, Mar. 22, 2019], 特許第6498412号（日本）
- [Registered, Feb. 15, 2019], 特許第6479154号（日本）
- [Registered, Dec. 7, 2018], 特許第6442941号（日本）
- [Registered, Jul. 13, 2018], 特許第6368318号（日本）
- [Registered, Jan. 19, 2018], 特許第6274570号（日本）
- [Registered, Oct. 2, 2015], 特許第5812478号（日本）
- [Registered, Jan. 14, 2015], ZL201080035929.8（中国）
- [Registered, Apr. 25, 2014], 特許第5526443号（日本）
- [Registered, Mar. 14, 2014], 特許第5499037号（日本）
- [Registered, Jan. 7, 2014], US8853373（米国）
- [Registered, Sep. 17, 2013], 特許第5357883号（日本）
- [Registered, Nov. 12, 2010], 特許第4621921号（日本）
- [Registered, May 9, 2008], 特許第4119976号（日本）
メディア報道
- ★経済再開へ“開国”のカギは？(テレビ東京・ワールドビジネスサテライト)
テレビ東京, ワールドビジネスサテライト, 2020年06月25日, テレビ・ラジオ番組 - ★塩野義、コロナ30分判定キット開発へ 日大などと契約(日本経済新聞・朝刊・第1面)
日本経済新聞, 2020年06月22日, 新聞・雑誌 - ★バイオマーカー簡便に検出 日大、試薬に入れるだけ(日経産業新聞・朝刊・第6面)
日経産業新聞（日本経済新聞社）, 2020年01月14日, 新聞・雑誌 - 30分で感染判定 新検査法を開発(朝日新聞・朝刊・第16面)
朝日新聞, 2020年07月01日, 新聞・雑誌 - コロナ新診断法実用化へ 塩野義製薬と契約(上毛新聞・日刊・第1面)
上毛新聞, 2020年06月23日, 新聞・雑誌 - 感染検査「SATIC法」実用化へ PCRと変わらない精度(テレ朝ニュース)
テレビ朝日, テレ朝ニュース, 2020年06月22日, テレビ・ラジオ番組 - 新型コロナ 30分以内に感染判定 新検査法開発 実用化へ(NHKニュース)
NHK, NHKニュース, 2020年06月22日, テレビ・ラジオ番組 - コロナ感染に新診断法(上毛新聞・日刊・第18面)
上毛新聞, 2020年05月20日, 新聞・雑誌
