PhD, Okayama National University, Mar. 1999

Isothermal Signal Amplification

Molecular Probe

Xeno Nucleic Acid

Nucleic Acid Aptamer

Bioanalysis

Nanotechnology/Materials, Biochemistry, Bio-related Chemistry

Nanotechnology/Materials, Molecular biochemistry, Biomolecular Chemistry

Nanotechnology/Materials, Chemical biology, Chemical Biology

Nanotechnology/Materials, Analytical chemistry, Analytical Chemistry

Apr. 2018 - Present
Nihon University, Department of Chemistry, College of Humanities and Sciences, Professor

Apr. 2013 - Mar. 2018
Gunma University, National University Corporation, Graduate School of Science and Technology, Associate Professor

Dec. 2009 - Mar. 2013
Gunma University, National University Corporation, Graduate School of Engineering, Associate Professor

Apr. 2007 - Nov. 2009
Gunma University, National University Corporation, Graduate School of Engineering, Assistant Professor

Oct. 2004 - Mar. 2008
Japan Science and Technology Agency (JST), Strategic Basic Research Program (PRESTO), PRESTO Researcher (concurrent post)

Apr. 2004 - Mar. 2007
Gunma University, National University Corporation, Department of Applied Chemistry, Faculty of Engineering, Research Associate

Jun. 2001 - Mar. 2004
Gunma National University, Department of Applied Chemistry, Faculty of Engineering, Research Associate

Apr. 2000 - May 2001
University of Virginia (USA), Department of Chemistry, Research Associate

Apr. 1999 - Mar. 2000
Okayama National University, Faculty of Engineering, Adjunct Lecturer

Mar. 1999
Okayama National University, Graduate School of Natural Science and Technology, Doctoral Program

Mar. 1996
Okayama National University, Graduate School of Engineering, Master Degree Program

Mar. 1994
Okayama National University, Faculty of Engineering, Department of Biological Functions Engineering

Minister of Education, Culture, Sports, Science and Technology Commendation Young Scientists' Prize

★DNA polymerase variants with high processivity and accuracy for encoding and decoding locked nucleic acid sequences
Hidekazu Hoshino; Yuuya Kasahara; Masayasu Kuwahara; Satoshi Obika
Journal of the American Chemical Society, Dec. 2020
Corresponding

★Seconds-resolved pharmacokinetic measurements of the chemotherapeutic irinotecan: In situ in the living body
Andrea Idili; Netzahualcóyotl Arroyo-Currás; Kyle L. Ploense; Andrew T. Csordas; Masayasu Kuwahara; Tod E. Kippin; Kevin W. Plaxco
Chemical Science, 2019

★In situ condensation of an anti-cancer drug into fibrin gel enabling effective inhibition of tumor cell growth
Masayasu Kuwahara; Hiroto Fujita; Yuka Kataoka; Yasuyo Nakajima; Masanobu Yamada; Naoki Sugimoto
Chemical Communications, 2019
Lead

★Novel one-tube-one-step real-time methodology for rapid transcriptomic biomarker detection: Signal amplification by ternary initiation complexes
Hiroto Fujita; Yuka Kataoka; Seiji Tobita; Masayasu Kuwahara; Naoki Sugimoto
Analytical Chemistry, Jul. 2016
Corresponding

★Minimal thioflavin T modifications improve visual discrimination of guanine-quadruplex topologies and alter compound-induced topological structures
Yuka Kataoka; Hiroto Fujita; Yuuya Kasahara; Toshitada Yoshihara; Seiji Tobita; Masayasu Kuwahara
Analytical Chemistry, Dec. 2014
Corresponding

★Identification and codon reading properties of 5-cyanomethyl uridine, a new modified nucleoside found in the anticodon wobble position of mutant haloarchaeal isoleucine tRNAs
Debabrata Mandal; Caroline Köhrer; Dan Su; I. Ramesh Babu; Clement T.Y. Chan; Yuchen Liu; Dieter Söll; Paul Blum; Masayasu Kuwahara; Peter C. Dedon; Uttam L. Rajbhandary
RNA, Feb. 2014

★Efficacy of base-modification on target binding of small molecule DNA aptamers
Yuri Imaizumi; Yuuya Kasahara; Hiroto Fujita; Shunsuke Kitadume; Hiroaki Ozaki; Tamaki Endoh; Masayasu Kuwahara; Naoki Sugimoto
Journal of the American Chemical Society, Jun. 2013
Corresponding

★Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection of base- and sugar-modified DNA aptamers: Target binding dominated by 2'- O,4'-C-methylene-bridged/locked nucleic acid primer
Yuuya Kasahara; Yuuta Irisawa; Hiroto Fujita; Aiko Yahara; Hiroaki Ozaki; Satoshi Obika; Masayasu Kuwahara
Analytical Chemistry, May 2013
Corresponding

★Artificially created nucleic acids and peptides/proteins in chemical biology
Masayasu Kuwahara; Yingfu Li; Eriks Rozners; Hiroshi Murakami
Journal of Nucleic Acids, 2013
Lead

★Systematic analysis of enzymatic DNA polymerization using oligo-DNA templates and triphosphate analogs involving 2',4'-bridged nucleosides
Masayasu Kuwahara; Satoshi Obika; Jun Ichi Nagashima; Yuki Ohta; Yoshiyuki Suto; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi
Nucleic Acids Research, Aug. 2008
Lead

★Modified DNA aptamer that binds the (R)-isomer of a thalidomide derivative with high enantioselectivity
Atsushi Shoji; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Journal of the American Chemical Society, Feb. 2007

★Influence of cationic molecules on the hairpin to duplex equilibria of self-complementary DNA and RNA oligonucleotides
Shu Ichi Nakano; Toshimasa Kirihata; Satoshi Fujii; Hiroshi Sakai; Masayasu Kuwahara; Hiroaki Sawai; Naoki Sugimoto
Nucleic Acids Research, Jan. 2007

★Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA
Masayasu Kuwahara; Jun Ichi Nagashima; Masatoshi Hasegawa; Takehiro Tamura; Rina Kitagata; Kazuo Hanawa; Shin Ichi Hososhima; Toshiyuki Kasamatsu; Hiroaki Ozaki; Hiroaki Sawai
Nucleic Acids Research, Nov. 2006
Lead

★Nucleic acid with guanidinium modification exhibits efficient cellular uptake
Tatsuo Ohmichi; Masayasu Kuwahara; Naoko Sasaki; Masatoshi Hasegawa; Takahito Nishikata; Hiroaki Sawai; Naoki Sugimoto
Angewandte Chemie - International Edition, Oct. 2005

★Design and optimization of camptothecin conjugates of triple helix-forming oligonucleotides for sequence-specific DNA cleavage by topoisomerase I
Paola B. Arimondo; Alexandre Boutorine; Brigitte Baldeyrou; Christian Bailly; Masayasu Kuwahara; Sidney M. Hecht; Jian Sheng Sun; Thérèse Garestier; Claude Hélène
Journal of Biological Chemistry, Feb. 2002

★Hybridization between oxy-peptide nucleic acids and DNAs: Dependence of hybrid stabilities on the chain-lengths, types of base pairs, and the chain directions
Masayasu Kuwahara, Miki Arimitsu, Masanori Shigeyasu, Naho Saeki, Masahiko Sisido
Journal of the American Chemical Society, 2001
Lead

★Novel peptide nucleic acid that shows high sequence specificity and all- or-none-type hybridization with the complementary DNA
Masayasu Kuwahara; Miki Arimitsu; Masahiko Sisido
Journal of the American Chemical Society, Jan. 1999
Lead

Aptamer-based biosensors for wastewater surveillance of influenza virus, SARS-CoV-2, and norovirus: A comprehensive review
Yilei Wang, Mohan Amarasiri, Wakana Oishi, Masayasu Kuwahara, Yuka Kataoka, Hiroki Kurita, Fumio Narita, Rong Chen, Qian Li, Daisuke Sano
Water Research, Mar. 2025

ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead
Masumi Taki; Masayasu Kuwahara; Chaohui Li; Naoko Tomoda; Naoyuki Miyashita; Tetsuo Kan; Jay Yang
Chemical Communications, Dec. 2024

Lantern-type G-quadruplex fluorescent sensors for detecting divalent metal ions
Tomoko Wariishi; Yuka Kataoka; Tomoaki Nakamura; Yuuya Kasahara; Masataka Kuroda; Satoshi Obika; Masayasu Kuwahara
Analytical Biochemistry, Jul. 2024

Field-effect transistor biosensor with signal amplification by ternary initiation complexes for detection of wide-range RNA concentration.
Hiroki Hayashi; Akihiro Enami; Hiroto Fujita; Shigeki Kuroiwa; Keishi Ohashi; Masayasu Kuwahara; Tetsuya Osaka; Toshiyuki Momma
Talanta, Jun. 2024

Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method
Kunihiko Morihiro; Osamu Hasegawa; Yuuya Kasahara; Shohei Mori; Tatsuro Kasai; Masayasu Kuwahara; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, Jan. 2021

Bifunctional aptamer drug carrier enabling selective and efficient incorporation of an approved anticancer drug irinotecan to fibrin gels
Hiroto Fujita; Yuka Kataoka; Masayasu Kuwahara
Applied Sciences (Switzerland), Dec. 2020

Effects of Modifying Thioflavin T at the N³-Position on Its G4 Binding and Fluorescence Emission
Yuka Kataoka; Hiroto Fujita; Tamaki Endoh; Naoki Sugimoto; Masayasu Kuwahara
Molecules (Basel, Switzerland), Oct. 2020

A high affinity modified DNA aptamer containing base-appended bases for human β-defensin
Hirotaka Minagawa; Yuka Kataoka; Masayasu Kuwahara; Katsunori Horii; Ikuo Shiratori; Iwao Waga
Analytical Biochemistry, Apr. 2020

Modified DNA aptamers for C-reactive protein and lactate dehydrogenase-5 with sub-nanomolar affinities
Hirotaka Minagawa; Yuka Kataoka; Hiroto Fujita; Masayasu Kuwahara; Katsunori Horii; Ikuo Shiratori; Iwao Waga
International Journal of Molecular Sciences, Apr. 2020

Fluorescence Polarization-Based Rapid Detection System for Salivary Biomarkers Using Modified DNA Aptamers Containing Base-Appended Bases
Hirotaka Minagawa; Akihisa Shimizu; Yuka Kataoka; Masayasu Kuwahara; Shintaro Kato; Katsunori Horii; Ikuo Shiratori; Iwao Waga
Analytical Chemistry, Jan. 2020

High-Contrast Facile Imaging with Target-Directing Fluorescent Molecular Rotors, the N³-Modified Thioflavin T Derivatives
Yuka Kataoka; Hiroto Fujita; Arina Afanaseva; Chioko Nagao; Kenji Mizuguchi; Yuuya Kasahara; Satoshi Obika; Masayasu Kuwahara
Biochemistry, Feb. 2019

Selective incorporation of foreign functionality into fibrin gels through a chemically modified DNA aptamer
Hiroto Fujita; Yusuke Inoue; Masayasu Kuwahara
Bioorganic and Medicinal Chemistry Letters, Jan. 2018

Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1
Keisuke Tanaka; Yuuya Kasahara; Yoichi Miyamoto; Okuda Takumi; Tatsuro Kasai; Kentaro Onodera; Masayasu Kuwahara; Masahiro Oka; Yoshihiro Yoneda; Satoshi Obika
Organic and Biomolecular Chemistry, 2018

Synthesis and properties of microenvironment-sensitive oligonucleotides containing a small fluorophore, 3-aminobenzonitrile or 3-aminobenzoic acid
Hiroaki Ozaki; Takeshi Kawai; Masayasu Kuwahara
Tetrahedron, Dec. 2017

Specific light-up system for protein and metabolite targets triggered by initiation complex formation
Hiroto Fujita; Yuka Kataoka; Remi Nagano; Yasuyo Nakajima; Masanobu Yamada; Naoki Sugimoto; Masayasu Kuwahara
Scientific Reports, Dec. 2017

Selection, Characterization and Application of Artificial DNA Aptamer Containing Appended Bases with Sub-nanomolar Affinity for a Salivary Biomarker
Hirotaka Minagawa; Kentaro Onodera; Hiroto Fujita; Taiichi Sakamoto; Joe Akitomi; Naoto Kaneko; Ikuo Shiratori; Masayasu Kuwahara; Katsunori Horii; Iwao Waga
Scientific Reports, Mar. 2017

Real-Time Monitoring of G-Quadruplex Formation during Transcription
Tamaki Endoh; Ambadas B. Rode; Shuntaro Takahashi; Yuka Kataoka; Masayasu Kuwahara; Naoki Sugimoto
Analytical Chemistry, Feb. 2016

Consecutive incorporation of functionalized nucleotides with amphiphilic side chains by novel KOD polymerase mutant
Hidekazu Hoshino; Yuuya Kasahara; Hiroto Fujita; Masayasu Kuwahara; Kunihiko Morihiro; Shin Ichi Tsunoda; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, Jan. 2016

Non-Equilibrium Capillary Electrophoresis of Equilibrium Mixtures-Based Affinity Separation and Selective Enrichment of a Long-Length DNA Aptamer
Kenta Hagiwara; Yuuya Kasahara; Hiroto Fujita; Masayasu Kuwahara
Australian Journal of Chemistry, 2016

Selection of natural and base-modified DNA aptamers for a camptothecin derivative
Hiroto Fujita; Masayasu Kuwahara
Current Protocols in Nucleic Acid Chemistry, 2016

Polymerase incorporation of a 2'-deoxynucleoside-5'-triphosphate bearing a 4-hydroxy-2-mercaptobenzimidazole nucleobase analogue
Kunihiko Morihiro; Hidekazu Hoshino; Osamu Hasegawa; Yuuya Kasahara; Kohsuke Nakajima; Masayasu Kuwahara; Shin Ichi Tsunoda; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, Jun. 2015

Polymerase-mediated high-density incorporation of amphiphilic functionalities into DNA: Enhancement of nuclease resistance and stability in human serum
Hiroto Fujita; Kohsuke Nakajima; Yuuya Kasahara; Hiroaki Ozaki; Masayasu Kuwahara
Bioorganic and Medicinal Chemistry Letters, Jan. 2015

In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids
Kenta Hagiwara; Hiroto Fujita; Yuuya Kasahara; Yuuta Irisawa; Satoshi Obika; Masayasu Kuwahara
Molecular BioSystems, Jan. 2015

Creation of nucleic acid aptamers that contain unnatural nucleotides
Masayasu Kuwahara
Kobunshi, Oct. 2014

2',4'-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides
Yuuya Kasahara; Yuuta Irisawa; Hiroaki Ozaki; Satoshi Obika; Masayasu Kuwahara
Bioorganic and Medicinal Chemistry Letters, Mar. 2013

Cleavage of supercoiled plasmid DNA by phenanthrolinepolyamine conjugates as a metal-free artificial nuclease
Tomokazu Masuda; Takeshi Sasagase; Akiko N. Ozaki; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Chemistry Letters, Jan. 2013

Structural and affinity analyses of G-quadruplex DNA aptamers for camptothecin derivatives
Hiroto Fujita; Yuri Imaizumi; Yuuya Kasahara; Shunsuke Kitadume; Hiroaki Ozaki; Masayasu Kuwahara; Naoki Sugimoto
Pharmaceuticals, 2013

In vitro selection of BNA (LNA) aptamers
Masayasu Kuwahara; Satoshi Obika
Artificial DNA: PNA and XNA, 2013

Specific amino acid sensing using a single acridone-labeled DNA aptamer
Hiroaki Ozaki; Yasuhisa Hagiwara; Masayasu Kuwahara
Chemistry Letters, 2012

Artificial specific binders directly recovered from chemically modified nucleic acid libraries
Yuuya Kasahara; Masayasu Kuwahara
Journal of Nucleic Acids, 2012

Optimization of pyrosequencing reads by superior successive incorporation efficiency of improved 2'-deoxyadenosine-5'-triphosphate analogs
Tomoharu Kajiyama; Masayasu Kuwahara; Mari Goto; Hideki Kambara
Analytical Biochemistry, Sep. 2011

Synthesis of modified siRNA bearing C-5 polyamine-substituted pyrimidine nucleoside in their 3'-overhang regions and its RNAi activity
Mohammad Mehedi Masud; Tomokazu Masuda; Yusuke Inoue; Masayasu Kuwahara; Hiroaki Sawai; Hiroaki Ozaki
Bioorganic and Medicinal Chemistry Letters, Jan. 2011

Nucleotide modification and polymerase engineering for creating a novel class of artificial nucleic acid aptamers
Y. Imaizumi; Y. Takano; S. Kitadume; H. Ozaki; S. Obika; M. Kuwahara
IFMBE Proceedings, 2011

Study on suitability of kod dna polymerase for enzymatic production of artificial nucleic acids using base/sugar modified nucleoside triphosphates
Masayasu Kuwahara; Yuuki Takano; Yuuya Kasahara; Hiroki Nara; Hiroaki Ozaki; Hiroaki Sawai; Akio Sugiyama; Satoshi Obika
Molecules, Nov. 2010

Molecular evolution of functional nucleic acids with chemical modifications
Masayasu Kuwahara; Naoki Sugimoto
Molecules, Aug. 2010

Effect of 3'-end capping of aptamer with various 2',4'-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers
Yuuya Kasahara; Shunsuke Kitadume; Kunihiko Morihiro; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi; Satoshi Obika
Bioorganic and Medicinal Chemistry Letters, Mar. 2010

Polymerase reactions using artificial nucleic acids and creation of artificial nucleic acid aptamers
Masayasu Kuwahara
Seikagaku, 2010

Synthesis and properties of oligodeoxyribonucleotides bearing a polyamino group at the 2' position via 2'-O-carbamoylmethyl and 2'-S-carbamoylmethyl groups
Hiroaki Ozaki; Shingo Nomura; Shingo Momiyama; Kazuyuki Yokotsuka; Masayasu Kuwahara; Hiroaki Sawai
Nucleosides, Nucleotides and Nucleic Acids, Oct. 2009

Smart conferring of nuclease resistance to DNA by 3'-end protection using 2',4'-bridged nucleoside-5'-triphosphates
Masayasu Kuwahara; Satoshi Obika; Hidetoshi Takeshima; Yoshihiko Hagiwara; Jun ichi Nagashima; Hiroaki Ozaki; Hiroaki Sawai; Takeshi Imanishi
Bioorganic and Medicinal Chemistry Letters, Jun. 2009

Fluorescent properties of acridonyl group in DNA duplex.
Masayasu Kuwahara; Atsushi Kobayashi; Masayoshi Tajima; Shunsuke Kitazume; Keisuke Anahara; Hiroaki Ozaki; Seiji Tobita
Nucleic acids symposium series (2004), 2009

Acridone-labeled DNA aptamer for the detection of biomolecules.
Hiroaki Ozaki; Yasuhisa Hagiwara; Hiromi Asakura; Masayasu Kuwahara
Nucleic acids symposium series (2004), 2009

Acridone-tagged DNA as a new probe for DNA detection by fluorescence resonance energy transfer and for mismatch DNA recognition
Yasuhisa Hagiwara; Tomoya Hasegawa; Atushi Shoji; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry, Jul. 2008

Conformation and the sodium ion condensation on DNA and RNA structures in the presence of a neutral cosolute as a mimic of the intracellular media
Shu-ichi Nakano; Lei Wu; Hirohito Oka; Hisae Tateishi Karimata; Toshimasa Kirihata; Yuichi Sato; Satoshi Fujii; Hiroshi Sakai; Masayuki Kuwahara; Hiroaki Sawai; Naoki Sugimoto
Molecular BioSystems, 2008

Arginine-modified DNA aptamers that show enantioselective recognition of the dicarboxylic acid moiety of glutamic acid
Kazuomi Ohsawa; Toshiyuki Kasamatsu; Jun Ichi Nagashima; Kazuo Hanawa; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Analytical Sciences, Jan. 2008

Effect of backbone-modification of oligodeoxyribonucleic acid on primer extension reactions.
Masayasu Kuwahara; Satoshi Minezaki; Jun ichi Nagashima; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2008

Synthesis and incorporation of fluorescent C-nucleoside analogue into oligodeoxyribonucleotides.
Hiroaki Ozaki; Takeshi Kawai; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2008

Chemico-enzymatic synthesis of a new fluorescent-labeled DNA by PCR with a thymidine nucleotide analogue bearing an acridone derivative
Atsushi Shoji; Tomoya Hasegawa; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, Feb. 2007

Polymerisation of a DNA strand using oligo-DNA template with modified bases, sugars and phosphates.
Jun Ichi Nagashima; Satoshi Minezaki; Satoshi Obika; Takeshi Imanishi; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2007

Synthesis of novel fluorescent nucleoside bearing 3-aminobenzonitrile as a nucleobase.
Hiroaki Ozaki; Takahiro Setaka; Syouta Tsuchiya; Takeshi Kawai; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2007

Interaction with and cleavage of DNA by novel phenanthroline polyamine derivative.
Takeshi Sasagase; Takeshi Arai; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2007

Differences in substrate specificity of C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides by DNA polymerases from thermophilic bacteria, archaea, and phages
Hiroaki Sawai; Junichi Nagashima; Msayasu Kuwahara; Rina Kitagata; Takehiro Tamura; Ikuo Matsui
Chemistry and Biodiversity, 2007

Biomolecular sensor based on fluorescence-labeled aptamer
Hiroaki Ozaki; Akifumi Nishihira; Masayuki Wakabayashi; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, Aug. 2006

Direct PCR amplification of various modified DNAs having amino acids: Convenient preparation of DNA libraries with high-potential activities for in vitro selection
Masayasu Kuwahara; Kazuo Hanawa; Kazuomi Ohsawa; Rina Kitagata; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry, Apr. 2006

Substrate property and incorporation accuracy of various dATP analogs during enzymatic polymerization using thermostable DNA polymerases.
Masayasu Kuwahara; Yoshiyuki Suto; Satoshi Minezaki; Rina Kitagata; Jun ichi Nagashima; Hiroaki Sawai
Nucleic acids symposium series (2004), 2006

Synthesis and property of DNA labeled with fluorescent acridone.
Tomoya Hasegawa; Atsusi Shoji; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2006

Assembly of DNA nanostructures with branched tris-DNA
Takahiro Kuroda; Yoshimasa Sakurai; Yukinao Suzuki; Akiko O. Nakamura; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Chemistry - An Asian Journal, 2006

Expansion of repertoire of modified DNAs prepared by PCR using KOD Dash DNA polymerase
Tsutomu Ohbayashi; Masayasu Kuwahara; Masatoshi Hasegawa; Toshiyuki Kasamatsu; Takehiro Tamura; Hiroaki Sawai
Organic and Biomolecular Chemistry, Jul. 2005

Screening of a glutamic acid-binding aptamer from arginine-modified DNA library.
Masayasu Kuwahara; Kazuomi Ohsawa; Toshiyuki Kasamatsu; Atsushi Shoji; Hiroaki Sawai; Hiroaki Ozaki
Nucleic acids symposium series (2004), 2005

Coformational switch of oligonucleotide induced by spermine.
Toshimasa Kirihata; Shu ichi Nakano; Satoshi Fujii; Manabu Matsumoto; Masayasu Kuwahara; Hiroaki Sawai; Naoki Sugimoto
Nucleic acids symposium series (2004), 2005

Comparison study on PCR amplification of modified DNA by using various kinds of polymerase and modified nucleoside triphosphates.
Masayasu Kuwahara; Takehiro Tamura; Rina Kitagata; Hiroaki Sawai; Hiroaki Ozaki
Nucleic acids symposium series (2004), 2005

Synthesis and properties of oligonucleotides containing 5-substituted pyrimidine nucleoside
Hiroaki Ozaki; Masayasu Kuwahara; Hiroaki Sawai
Yuki Gosei Kagaku Kyokaishi/Journal of Synthetic Organic Chemistry, Dec. 2004

Synthesis and antisense properties of oligodeoxyribonucleotides containing C5-substituted arabinofuranosyluracil
Hiroaki Ozaki; Kiyohiro Nakajima; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, Mar. 2004

Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX
Mohammad Mehedi Masud; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry, Mar. 2004

Detection of biomolecule by aptamer beacon.
Akifumi Nishihira; Hiroaki Ozaki; Masayuki Wakabayashi; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids symposium series (2004), 2004

Screening of modified DNA aptamers that recognize DNA secondary structure.
Masayasu Kuwahara; Toshiyuki Kasamatsu; Masatoshi Hasegawa; Atsushi Shoji; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids symposium series (2004), 2004

Substrate properties of C5-substituted pyrimidine 2'-deoxynucleoside 5'-triphosphates for thermostable DNA polymerases during PCR
Masayasu Kuwahara; Yumi Takahata; Atsushi Shoji; Akiko N. Ozaki; Hiroaki Ozaki; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, Nov. 2003

Convenient synthesis of oligodeoxyribonucleotides bearing arabinofuranosyl pyrimidine derivatives and its duplex formation with complementary DNA
Hiroaki Ozaki; Kiyohiro Nakajima; Kaoru Tatsui; Chieko Izumi; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, Aug. 2003

Modified DNA bearing 5-(methoxycarbonyl-methyl)-2′-deoxyuridine: Preparation by PCR with thermophilic DNA polymerase and post-synthetic derivatization
Mohammad Mehedi Masud; Akiko Ozaki-Nakamura; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
ChemBioChem, Jul. 2003

Simultaneous incorporation of three different modified nucleotides during PCR.
Masayasu Kuwahara; Shin ichi Hososhima; Yumi Takahata; Rina Kitagata; Atsushi Shoji; Kazuo Hanawa; Akiko N. Ozaki; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2003

Synthesis and properties of oligonucleotides containing C-2 and/or C-5 substituted arabinofuranosyluracils.
Hiroaki Ozaki; Kiyohiro Nakajima; Kyoko Yajima; Kaoru Tatsui; Masayasu Kuwahara; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2003

Enzymatic synthesis of labeled DNA by PCR using new fluorescent thymidine nucleotide analogue and superthermophilic KOD dash DNA polymerase
Tsutomu Obayashi; Mohammed M. Masud; Akiko N. Ozaki; Hiroaki Ozaki; Masayasu Kuwahara; Hiroaki Sawai
Bioorganic and Medicinal Chemistry Letters, Apr. 2002

Enzymatic incorporation of chemically-modified nucleotides into DNAs.
Masayasu Kuwahara; Tsutomu Ohbayashi; Kazuo Hanawa; Atsushi Shoji; Akiko N. Ozaki; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2002

Modified DNA aptamers against sweet agent aspartame.
Hiroshi Saitoh; Akiko Nakamura; Masayasu Kuwahara; Hiroaki Ozaki; Hiroaki Sawai
Nucleic acids research. Supplement (2001), 2002

Synthesis and DNA binding property of a novel peptide nucleic acid that contains cis-4-adeninyl-L-prolinol unit
Masanori Shigeyasu; Masayasu Kuwahara; Masahiko Sisido; Teruhiko Ishikawa
Chemistry Letters, 2001

Synthesis of δ-amino acids with an ether linkage in the main chain and nucleobases on the side chain as monomer units for oxy-peptide nucleic acids
Masayasu Kuwahara; Miki Arimitsu; Masahiko Sisido
Tetrahedron, Aug. 1999

Sequence-specific hybridization between two different types of peptide nucleic acids
Masayasu Kuwahara; Miki Arimitsu; Masahiko Sisido
Bulletin of the Chemical Society of Japan, Jul. 1999

Synthesis of oxy-peptide nucleic acids with mixed sequences.
M. Kuwahara; M. Arimitsu; M. Sigeyasu; M. Sisido
Nucleic acids symposium series, 1999

Peptide Nucleic Acid with Ether Linkages in the Main Chain. Synthesis and Sequence-Specific Interaction with DNA
Masahiko Sisido, Miki Arimitsu, Masayasu Kuwahara
Oct. 1998

★Handbook of chemical biology of nucleic acids (edited by Naoki Sugimoto)
Fujita H; Kuwahara M, Contributor, Detection systems using the ternary complex formation of nucleic acids
Springer, Singapore, Oct. 2022
9789811613135

★Modified nucleic acids in biology and medicine (RNA technologies) (edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski)
Kuwahara M, Hagiwara K, Ozaki H, Contributor, Polymerase reactions that involve modified nucleotides
Springer, Heidelberg, Aug. 2016
9783319341736

★Chemical biology of nucleic acids: Fundamentals and clinical applications (RNA technologies) (edited by Volker A. Erdmann, Wojciech T. Markiewicz, Jan Barciszewski)
Kuwahara M, Contributor, Progress in chemically modified nucleic acid aptamers
Springer, Heidelberg, May 2014
9783642544514

★DNA sequencing–methods and apprications (edited by Anjana Munshi)
Kuwahara M; Sugimoto N, Contributor, Nucleic acid aptamers as molecular tags for omics analyses involving sequencing
INTECH-Open Access Publisher, Croatia, Apr. 2012
9535105647

CSJ current review: Evolving nucleic acid chemistry (edited by Chemical Society of Japan)
Kataoka Y; Kuwahara M, Contributor, Part II Research frontline (Chapter 5) Progress in nucleic acid aptamer development with chemical modification
Kagaku-Dojin Publishing Co., Inc., Oct. 2021
9784759814019

Handbook for nucleic acid science (supervised by Japan Society of Nucleic Acids Chemistry, edited by Naoki Sugimoto)
Kuwahara M, Contributor, Part II Forefront of nucleic acid science (23) Simple detection method for nucleic acid strand-related biomarkers
Kodansha Scientific, Ltd., Dec. 2020
9784065207864

CSJ current review: Controlling biomolecular reactions (edited by Chemical Society of Japan)
Kuwahara M; Sugimoto N, Contributor, Part II Frontline of research (Chapter 11) Nucleic acid function control by phase separation and molecular crowding
Kagaku-Dojin Publishing Co., Inc., Apr. 2020
9784759813968

Synthesis technology of peptides, nucleic acids and sugar chains for medium molecule drug discovery (supervised by Kazuhiro Chiba)
Ozaki H, Hagiwara K, Kuwahara M, Contributor, Development of xeno-nucleic acid aptamers
CMC Publishing CO., LTD., Feb. 2018
9784781313207

CSJ current review: New trends in nucleic acid chemistry (edited by Chemical Society of Japan)
Kuwahara M, Contributor, Part II Research front (Chapter 5) Nucleic acid aptamers
Kagaku-Dojin Publishing Co., Inc., Jul. 2011
9784759813661

Forefront of single cell analysis (edited by Hideki Kambara, Tadashi Matsunaga, Mitsubayashi Ueda)
Kuwahara M, Kajiyama T., Contributor, (4.5) Polymerase reaction system using modified substrate analogs and methods of DNA sequence analysis
CMC Publishing CO., LTD., Mar. 2010
9784781302034

Frontiers in organic chemistry. Volume 1 (edited by Atta-ur-Rahman, Yoshihiro Hayakawa)
Ozaki H; Kuwahara M; Sawai H, Contributor, Oligonucleotides bearing α5-substituted pyrimidine nucleoside: Their synthesis, properties, and application
Bentham Science Publishers Ltd., Sharjah., Mar. 2005
9077527060

Genome chemistry (edited by Mitsuo Sekine, Isao Saito)
Sawai H; Kuwahara M, Contributor, (4.2) Extension of in vitro selection method (SELEX method) using artificially modified nucleic acids
Kodansha Scientific, Ltd., Mar. 2003
9784061543157

Self-assembling peptide systems in biology, medicine and engineering (edited by Amalia Aggeli, Neville Boden, Shuguang Zhang)
Sisido M; Kuwahara M, Contributor, Novel peptide nucleic acids with improved solubility and DNA-binding ability
Kluwer Academic Publishers, Boston., Dec. 2001
9780792370901

★Enhanced selectivity over topology and altered targeting by fluorescent G4 binder modifications
Kuwahara M.
Asian 3 Roundtable on Nucleic Acids 2024 (A3RONA 2024), Nov 2, 2024, Nov. 2024, Invited

★Fluorescent binders with enhanced selectivity for G-quadruplex topologies
Kuwahara M
Advances in Noncanonical Nucleic Acids (ANNA 2024), Oct 26, 2024, Oct. 2024, Invited

★Halfway through the development of technology to create molecular recognition polymers that function in water
Kuwahara M
Supra FIBER International Summit for Nucleic Acids (S-FISNA), Feb. 2024, Invited

★Modified substrates and polymerase variants for functional xeno nucleic acid selections
Kuwahara M
FIBER International Summit for Nucleic Acids 2023 (FISNA2023), Jul. 2023, Invited

★Fluorescence molecular rotors Thioflavin T derivatives for various targets
Kuwahara M
The 2nd International Symposium on Functional Nucleic Acids (FNA2018), Nov. 2018, Invited

★Development of unique nucleic acid materials for diagnosis and therapeutics
Kuwahara M
NanoTech Poland 2018, Jun. 2018, Invited

★Xenonucleic acid aptamers: development towards in vitro selection of artificial biopolymer ligands/receptors
Kuwahara M
The 32nd International Symposium on Microscale Separations and Bioanalysis (MSB 2016), Apr. 2016, Invited

★Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection using modified nucleic acid libraries
Kuwahara M
2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015), Dec. 2015, Invited

★Functional nucleic acids with chemical modifications
Kuwahara M
POLYCHEM Seminar (Host: Prof. D.A.Schluter, Swiss Federal Institute of Technology Zurich (ETHZ)), Sep. 2011, Invited

Modified nucleic acid aptamer selections using polymerase variants and substrate analogs
Kuwahara M
Asian 3 Roundtable on Nucleic Acids 2023 (A3RONA 2023), Nov 11, 2023, Nov. 2023, Invited

Detection systems that start with the ternary initiation complex formation
Kuwahara M
FIBER International Summit for Nucleic Acids 2022 (FISNA2022), Jul. 2022, Invited

Xeno-nucleic acid aptamers: Progress in nucleic acid aptamer development with chemical modification
Kuwahara M
The 2nd Aptamer Seminar in Tokyo, Jul. 2021, Invited

Thioflavin T modification and the application to the detection of biomarkers and viruses
Kuwahara M
13th JKBT: Asian Workshop against Coronavirus Disease, Jan. 2021, Invited

Ultra-high sensitive simple test methods for various biomarkers
Kuwahara M
Advances in Noncanonical Nucleic Acids 2019 (ANNA2019), Oct. 2019, Invited

Method for high sensitive detection of various biomarkers
Kuwahara M
Asian 3 Roundtable on Nucleic Acids 2019 (A3RONA 2019), Oct. 2019, Invited

Simple test methods for various biomarkers at attomolar level
Kuwahara M
Poland-Japan RNA Meeting, Poznań 2019, Jun. 2019, Invited

Target-directing fluorescent molecular rotors
Kuwahara M
Asian 3 Roundtable on Nucleic Acids 2018 (A3RONA 2018), Sep. 2018, Invited

Simple method for various biomarker detections
Kuwahara M
Advances in Noncanonical Nucleic Acids (ANNA 2017), Oct. 2017, Invited

One-tube-one-step real-time quantitative system for various biomolecular targets
Kuwahara M
Asia 3 Roundtable on Nucleic Acids 2017 (A3RONA 2017), Sep. 2017, Invited

Specific light-up system for various biomarker detection
Kuwahara M
FIBER International Summit for Nucleic Acids 2017 (FISNA 2017), Jul. 2017, Invited

Simple method for biomarker detection: Signal amplification by ternary initiation complexes
Kuwahara M
XVIIth Symposium on Chemistry of Nucleic Acid Components, Jun. 2017, Invited

Signal amplification by ternary initiation complexes (SATIC) method for nucleic acid biomarker analyses
Kuwahara M
Japan-Spain Joint Workshop on Nanomedicine Research (AMED - MINECO – CSIC - ISCIII), Dec. 2016, Invited

Application of unnatural nucleic acids and non-canonical nucleic acid structures towards drug discovery and bioanalysis
Kuwahara M
2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015), Dec. 2015, Invited

Xeno-nucleic acid aptamers
Kuwahara M
The 1st International Symposium on “Functional Nucleic Acids: From Laboratory to Targeted Molecular Therapy” (FNA2015), Nov. 2015, Invited

Syntheses and properties of novel Thioflavin T analogs
Kuwahara M
Asia 3 Roundtable on Nucleic Acids 2015 (A3RONA 2015), Sep. 2015, Invited

Sep. 2017 - Present
Japan Society of Nucleic Acids Chemistry (JSNAC)

Nov. 2012 - Present
Japan Society for Analytical Chemistry (JSAC)

Aug. 2010 - Present
International Society for Nucleosides, Nucleotides and Nucleic Acids (IS3NA)

Apr. 2000 - Present
American Chemical Society (ACS)

Apr. 1999 - Present
Forum on Biomolecular Chemistry (FBC)

Apr. 1999 - Present
Society of Polymer Science, Japan (SPSJ)

Apr. 1999 - Present
Chemical Society of Japan (CSJ)

[Registered, Jan. 15, 2025], EP3957733（Germany, United Kingdom, France）

[Registered, October 3, 2024], No.7565572（Japan）

[Registered, Aug. 30, 2024], ZL202210227991.1（China）

[Registered, Mar. 4, 2024], No.7448239（Japan）

[Registered, May 9, 2023], US11643660（USA）

[Registered, Nov. 30, 2022], EP3546471（Germany, United Kingdom, France）

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[Registered, Sep. 27, 2022], ZL201780072997.3（China）

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[Registered, May 17, 2022], ZL201780056678.3（China）

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[Registered, Mar. 22, 2022], ZL201780056368.1（China）

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[Registered, Feb. 1, 2022], US11236342（USA）

[Registered, Jan. 31, 2022], No.7017246 (Japan)

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[Registered, Jan. 18, 2022], No.7011145 (Japan)

[Registered, Dec. 27, 2021], No.6999976 (Japan)

[Registered, Nov. 3, 2021], EP3783105 (Germany, United Kingdom, France), DE602017048961.1

[Registered, Oct. 19. 2021], No.6963221（Japan）

[Registered, Jul. 14, 2021], EP3514237 (Germany, United Kingdom, France), DE602017042271.1

[Registered, May 31, 2021], No.6892071 (Japan)

[Registered, May 19, 2021], EP3514165（Germany, United Kingdom, France）

[Registered, Jan. 19, 2021], No.6826275 (Japan)

[Registered, Nov. 16, 2020], No.6795211 (Japan)

[Registered, Sep. 22, 2020], US10781230（USA）

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[Registered, Sep. 20, 2020], No.6763553 (Japan)

[Registered, Sep. 9, 2020], EP3514164（Germany, United Kingdom, France）

[Registered, Sep. 1, 2020], US10760084（USA）

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[Registered, Apr. 7, 2020], US10611791（USA）

[Registered, Mar. 34, 2020], US10597719（USA）

[Registered, Aug. 20, 2019], US10385090（USA）

[Registered, Mar. 22, 2019], No.6498412 (Japan)

[Registered, Feb. 15, 2019], No.6479154 (Japan)

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[Registered, Oct. 2, 2015], No.5812478 (Japan)

[Registered, Jan. 14, 2015], ZL201080035929.8（China）

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[Registered, May 9, 2008], No.4119976（Japan）

★What is the key to "opening up" for economic resumption? (TV Tokyo, World Business Satellite)
TV Tokyo, World Business Satellite, 25 Jun. 2020, Media report

★Shionogi; to develop 30 minute corona diagnostic kit; contract with Nihon University etc. (Nihon Keizai Shimbun, Morning edition, First page)
Nihon Keizai Shimbun, 22 Jun. 2020, Paper

★Nihon University: Simple detection method for biomarkers, just put in reagents! (Nikkei Sangyo Shimbun, Morning edition, Page 6)
Nikkei Business Daily (Nikkei Inc.), 14 Jan. 2020, Paper

Infection determination in 30 minutes; development of new test method (Asahi Shimbun, Morning edition, Page 16)
Asahi Shimbun, 01 Jul. 2020, Paper

Practical application of new corona diagnostic method; Contract with Shionogi Pharma (Jomo Shimbun, Daily, First page)
Jomo Shimbun, 23 Jun. 2020, Paper

Practical use of infection test "SATIC method"; Accuracy as high as PCR (ANN News)
TV Asahi, ANN News, 22 Jun. 2020, Media report

New corona infection detection within 30 minutes; development of new test method for practical use (NHK News)
NHK, NHK News, 22 Jun. 2020, Media report

New diagnostic method for corona infection (Jomo Shimbun, Daily, Page 18)
Jomo Shimbun, 20 May 2020, Paper