Life sciences, Cell biology

A Set of Plasmid-Based Modules for Easy Switching of C-Terminal Epitope Tags in Saccharomyces cerevisiae
Hiroki Hayashi; Tsutomu Kishi
Microorganisms, Dec. 2021, Refereed

Switching off the genetic circuit that suppresses CDK activity
Switching off; he; genetic circuit that suppresses CDK activity; T. Kishi
化学系学協会, Sep. 2021, Not refereed, Invited

ARID2 is a pomalidomide-dependent CRL4CRBN substrate in multiple myeloma cells.
Junichi Yamamoto; Tetsufumi Suwa; Yuki Murase; Shumpei Tateno; Hirotaka Mizutome; Tomoko Asatsuma-Okumura; Nobuyuki Shimizu; Tsutomu Kishi; Shuji Momose; Masahiro Kizaki; Takumi Ito; Yuki Yamaguchi; Hiroshi Handa
Nature chemical biology, Nov. 2020, Refereed

Marker-free insertion of a series of C-terminal epitopes based on the 50:50 method in <i>Saccharomyces cerevisiae</i>
Masamichi Nonaka; Tsutomu Kishi
The Journal of General and Applied Microbiology, 2018, Refereed

New plasmids for the disruption and repeated use of selection markers in <i>Saccharomyces cerevisiae</i>
Kenshiro Nihei; Tsutomu Kishi
The Journal of General and Applied Microbiology, 2017, Refereed

SCFCdc4-dependent degradation of Swi5 is confined within a narrow window in G1 phase
H. Hayashi; T. Kishi
Genes Genet. Syst., Mar. 2015, Refereed

CDK is involved in the control of spindle checkpoint
N. Watanabe; H. Hayashi; S. Oku; T. Kishi
Genes Genet. Syst, Mar. 2015, Refereed

Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function.
Yuan Liu; Kunio Nakatsukasa; Michiko Kotera; Akira Kanada; Takashi Nishimura; Tsutomu Kishi; Satoru Mimura; Takumi Kamura
Molecular biology of the cell, May 2011, Refereed

Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Kanako Kume; Yosuke Iizumi; Masashi Shimada; Yuki Ito; Tsutomu Kishi; Yuki Yamaguchi; Hiroshi Handa
Genes to cells : devoted to molecular & cellular mechanisms, Apr. 2010, Refereed

SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast.
Satoru Mimura; Makiko Komata; Tsutomu Kishi; Katsuhiko Shirahige; Takumi Kamura
The EMBO journal, Dec. 2009, Refereed

A longevity protein, Lag2, interacts with SCF complex and regulates SCF function.
Yuan Liu; Satoru Mimura; Tsutomu Kishi; Takumi Kamura
The EMBO journal, Nov. 2009, Refereed

A refined two-hybrid system reveals that SCF(Cdc4)-dependent degradation of Swi5 contributes to the regulatory mechanism of S-phase entry.
Tsutomu Kishi; Akemi Ikeda; Noriko Koyama; Junji Fukada; Rina Nagao
Proceedings of the National Academy of Sciences of the United States of America, Sep. 2008, Refereed

Oxidative stress-induced ubiquitination of RCAN1 mediated by SCFbeta-TrCP ubiquitin ligase.
Sachie Asada; Akemi Ikeda; Rina Nagao; Hiroshi Hama; Tatsuhiko Sudo; Akiyoshi Fukamizu; Yoshitoshi Kasuya; Tsutomu Kishi
International journal of molecular medicine, Jul. 2008, Refereed

The SCFCdc4 ubiquitin ligase regulates calcineurin signaling through degradation of phosphorylated Rcn1, an inhibitor of calcineurin.
Tsutomu Kishi; Akemi Ikeda; Rina Nagao; Noriko Koyama
Proceedings of the National Academy of Sciences of the United States of America, Oct. 2007, Refereed

Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.
Hiroaki Seino; Tsutomu Kishi; Hideo Nishitani; Fumiaki Yamao
Molecular and cellular biology, May 2003, Refereed

An essential function of Grr1 for the degradation of Cln2 is to act as a binding core that links Cln2 to Skp1
Tsutomu Kishi; Fumiaki Yamao
Journal of Cell Science, Dec. 1998, Refereed

Grr1 functions in the ubiquitin pathway in Saccharomyces cerevisiae through association with Skp1
T. Kishi; T. Seno; F. Yamao
Molecular and General Genetics MGG, Jan. 1998, Refereed

Complementation assay of primer protein: Gene expression systems of plasmid vectors support the infection of suppressor sensitive mutant phages M2 and .PHI.29.
Tsutomu KISHI; Kin-ichiro MIURA; Kouji MATSUMOTO; Hideo HIROKAWA
The Japanese Journal of Genetics, 1993, Refereed

Mechanism of;rotein;m;DNA replication of;B.subtilis phage M2
T Kishi; K Miura; K Matsumoto; H Hirokawa
Nucleic Acids Symp Ser, Dec. 1992, Refereed

Regulation of Mphase progression by the SCFCdc4 ubiquitin ligase.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2011 - 2013
KISHI Tsutomu

The SCF ubiquitin ligase regulates calcineurin signaling through degradation of RCAN1, an inhibitor of calcineurin.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2008 - 2011
KISHI Tsutomu

ユビキチン化によって活性が制御される蛋白質のスクリーニングと機能解析
日本学術振興会, 科学研究費助成事業, 2002 - 2004
岸 努

SCFユビキチンリガーゼの標的蛋白質の網羅的スクリーニング
日本学術振興会, 科学研究費助成事業, 2002 - 2002
岸 努; 清野 浩明

SCFユビキチンリガーゼの標的蛋白質の網羅的スクリーニング
日本学術振興会, 科学研究費助成事業, 2001 - 2001
岸 努; 清野 浩明

ユビキチン系による染色体複製開始の制御
日本学術振興会, 科学研究費助成事業, 1998 - 1998
岸 努

Cellular functions regulated by specific ubiquitin pathways
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 1995 - 1997
YAMAO Fumiaki

G2-M期における核小体解離のユビキチン経路による制御
日本学術振興会, 科学研究費助成事業, 1995 - 1995
瀬野 悍二; 清野 浩明; 岸 努

核小体のG2-M期移行における代謝と動態のユビキチン系による制御
日本学術振興会, 科学研究費助成事業, 1995 - 1995
瀬野 悍二; 清野 浩明; 岸 努

ユビキチン系の制御を受けるDNA修復と誘導突然変異機構
日本学術振興会, 科学研究費助成事業, 1995 - 1995
瀬野 悍二; 清野 浩明; 岸 努

ユビキチン系酵素変異株を用いた哺乳類細胞の誘導性修復能の研究
日本学術振興会, 科学研究費助成事業, 1994 - 1994
瀬野 悍二; 岸 努