CHAEN Shigeru

College of Humanities and Sciences Department of BiosciencesProject Professor

Research Keyword

  • motor protein single-molecule analysis
  • Energy comversion
  • ATP
  • Kinesin
  • Myosin
  • Molecular motor
  • Biophysics

Field Of Study

  • Life sciences, Biophysics, Biophysics
  • Nanotechnology/Materials, Molecular biochemistry, Living Organism Molecular Science

Career

  • Apr. 2002 - Present
    日本大学文理学部物理・物理生命システム科学科教授
  • Apr. 2000 - Mar. 2002
    東京都臨床医学総合研究所主任研究員
  • Mar. 1994 - Mar. 2000
    帝京大学医学部第2生理学教室講師
  • Mar. 1992 - Feb. 1994
    アメリカ合衆国国立衛生研究所Visiting Scientist
  • Aug. 1985 - Feb. 1992
    帝京大学医学部第2生理学教室講師
  • Apr. 1980 - Jul. 1985
    帝京大学医学部第2生理学教室助手

Educational Background

  • 1980
    The University of Tokyo, Graduate School, Division of Pharmaceutical Sciences, Pharmaceutical Sciences
  • Apr. 1976 - Mar. 1978
    Kyushu University, Graduate School, Division of Natural Science, Biology
  • 1976
    Kyushu University, Faculty of Science, Biology

Award

  • 日本生物物理学会, BIOPHYSICS EDITORS' CHOICE AWARD 2014
    SINGLE TURNOVERS OF FLUORESCENT ATP BOUND TO BIPOLAR MYOSIN FILAMENT DURING ACTIN FILAMENTS SLIDING, Japan society
    TAKAHIRO MARUTA;TAKAHIRO KOBATAKE;HIROYUKI OKUBO;SHIGERU CHAEN

Paper

  • トリメチルアミンN-オキシドがミオシンの熱安定性に与える効果
    茶圓 茂、安田美咲、長友万柚子
    日本大学文理学部自然科学研究所研究紀要, Mar. 2022, Refereed, Not invited
    Lead
  • Both a unique motif at the C terminus and an N-terminal HEAT repeat contribute to G-quadruplex binding and origin regulation by the Rif1 protein
    Shunsuke Kobayashi; Rino Fukatsu; a Yutaka Kanoh; Naoko Kakusho; Seiji Matsumoto; Shigeru Chaen; Hisao Masaia
    Molecular and Cellular Biology, Feb. 2019, Refereed, Not invited
  • Possible cold-adaptation for the fungal kinesis in compensation for thermal stability acquired by single amino acid substitution
    Youske' Shimizu; Toru Togawa and Shigeru Chaen
    J. Biochem., Dec. 2018, Refereed, Not invited
  • Basic properties of ATP-induced myosin head movement in hydrated myosin filaments, studied using the gas environmental chamber
    H. Sugi; T. Akimoto; S. Chaen
    Micron, Jun. 2018, Refereed, Not invited
  • Electron Microscopic Recording of the Power and Recovery Strokes of Individual Myosin Heads Coupled with ATP Hydrolysis: Facts and Implications
    Haruo Sugi; Shigeru Chaen and Tsuyoshi Akimoto
    International Journal of Molecular Sciences, May 2018, Refereed, Invited
  • Mutations in the SH1 helix alter the thermal properties of myosin II
    Shibata K; Koyama T; Inde S; Iwai S; Chaen S
    Biophysics and Physicobiology, May 2017, Refereed, Not invited
    Corresponding
  • Force-Velocity Relation:Its Implication about Molecular Mechanism of Muscle Contraction
    Sugi H and Chaen S
    Journal of Nanomedicine & Nanotechnology, Oct. 2016, Refereed, Not invited
  • Tension Recovery following Ramp-Shaped Release in High-Ca and Low-Ca Rigor Muscle Fibers:Evidence for the Dynamic State of AMADP Myosin Heads in the Absence of ATP
    Sugi H; Yamaguchi M; Ohno T; Kobayashi T; Chaen S; Okuyama H
    PLOS ONE, Sep. 2016, Refereed, Not invited
  • Evidence against the Swinging Lever Arm Mechanism in Muscle Contraction Based on the Effect of Antibodies to Myosin Head.
    Sugi H; Chaen S
    J Nanomed Nanotechnol, May 2016, Refereed, Not invited
  • Relation between the molecular weight size of cargo and the nuclear transport time
    小畑貴広、赤塚拳斗、根本美薫、茶圓茂
    日本大学文理学部自然科学研究所研究紀要, Mar. 2016, Refereed, Not invited
    Corresponding
  • Comprehensive analysis of the dynamic structure of nuclear localization signals
    Yamagishi R; Okuyama T; Oba S; Shimada J; Chaen S; Kaneko H
    Biochemistry and Biophysics Reports, Nov. 2015, Refereed, Not invited
  • Electron microscopic recording of myosin head power stroke in hydrated myosin filaments
    Sugi H; Chaen S; Akimoto T; Minoda H; Miyakawa T; Miyauchi Y; Tanokura M; Sugiura S
    Scientific Reports, Oct. 2015, Not refereed, Not invited
  • Enhancement of Force Generated by Individual Myosin Heads in Skinned Rabbit Psoas Muscle Fibers at Low Ionic Strength
    Sugi H; Abe T; Kobayashi T; Chaen S; Ohnuki Y; Saeki Y; Sugiura S.
    PLoS One, May 2013, Refereed, Not invited
  • Single turnovers of fluorescent ATP bound to bipolar myosin filament during actin filaments sliding
    Maruta T; Kobatake T; Okubo H; Chaen S.
    BIOPHYSICS, Jan. 2013, Refereed, Not invited
    Corresponding
  • The gas environmental chamber as a powerful tool to study structural changes of living muscle thick filaments coupled with ATP hydrolysis
    Sugi H; Minoda H; Miyakawa T; Tanokura S; Chaen S; Kobayashi T
    INTECHOPEN.COM, Aug. 2012, Not refereed, Not invited
  • Evidence fro the essential role of myosin head lever arm domain and myosin subfragment-2 in muscle contraction
    Sugi H; Kobayashi T; Tsuchiya T; Chaen S; Sugiura S.
    INTECHOPEN.COM, Aug. 2012, Not refereed, Not invited
  • Thermal activation energy for bidirectional movement of actin along bipolar tracks of myosin filaments
    Hiroyuki Okubo; Masanori Iwai; Sosuke Iwai; Shigeru Chaen
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, May 2010, Refereed, Not invited
  • Kinesin-Calmodulin fusion protein as a molecular shuttle
    Hideki Shishido; Kiyoshi Nakazato; Eisaku Katayama; Shigeru Chaen; Shinsaku Maruta
    JOURNAL OF BIOCHEMISTRY, Feb. 2010, Refereed, Not invited
  • Direct demonstration of the cross-bridge recovery stroke in muscle thick filaments in aqueous solution by using the hydration chamber.
    Sugi; H.; Minoda; H.; Inayoshi; Y.; Yumoto; F.; Miyakawa; T.; Yumiko Miyauchi; Y.; Tanokura; M.; Akimoto; T.; Kobayashi; T.; Chaen; S.; and Sugiura; S.
    Proc. Natl. acad. Sci. USA, 2008, Refereed, Not invited
  • Mutation in the SH1 helix reduces the activation energy of the ATP-induced conformational transition of myosin
    Sosuke Iwai; Shigeru Chaen
    Biochemical and Biophysical Research Communications, 2007, Refereed, Not invited
  • ミオシンのATP依存的構造変化に含まれる拡散的な過程
    岩井草介、茶圓 茂
    日本大学文理学部自然科学研究所紀要, Mar. 2006, Refereed, Not invited
  • A point mutation in the SH1 helix alters elasticity and thermal staability of myosin II.
    Iwai; S.; Hanamoto; D.; and Chaen; S.
    Journal of Biological Chemistry, 2006, Refereed, Not invited
    Corresponding
  • 生体分子モーター1個の出す力を測る
    茶圓 茂
    日本大学文理学部化学科・自然科学研究所シンポジウム, Oct. 2005, Not refereed, Not invited
    Corresponding
  • Effect of deuterium oxide on contraction characteristics and ATPase activity in Glycerinated single rabbit skeletal muscle fibers.
    Kobayashi; T.; Saeki; Y.; Chaen; S.; Shirakawa; I. and Sugi; H.
    Biochimica et Biophysica Acta, Oct. 2004, Refereed, Not invited
    Lead
  • 筋肉の構造
    茶圓 茂
    Geriatric Med., Jul. 2004, Not refereed, Not invited
    Lead
  • 重水中のアクトミオシン滑り運動
    茶圓 茂
    日本大学文理学部自然科学研究所紀要, Mar. 2004, Refereed, Not invited
    Lead
  • 筋肉のマイクロ・ナノマシン技術
    茶圓 茂
    NSA Commentaries Series No. 11 [放射線と先端医療技術], Jun. 2003, Not refereed, Not invited
    Corresponding
  • Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells
    Sugi; H. and Chaen; S.
    J. Exp. Biol., Jun. 2003, Not refereed, Not invited
    Lead
  • Force-velocity relationships in actin-myosin interactions causing cytoplasmic streaming in algal cells
    Sugi, H; Chaen, S
    J. Exp. Biol., Jun. 2003, Not refereed, Not invited
  • In vitro actomyosin motility in deuterium oxide.
    Chaen; S.; Yamamoto; N.; Shirakawa; I. and Sugi. H.
    Adv. Exp. Med. Biol., 2003, Refereed, Not invited
    Lead
  • Interaction of a new fluorescent ATP analogue with skeletal muscle myosin subfragment-1.
    Maruta; S.; Mizukura; Y.; and Chaen; S.
    J. Biochem., 2002, Refereed, Not invited
  • Effect of deuterium oxide on actomyosin motility in vitro.
    Chaen; S.; Yamamoto; N.; Shirakawa; I. and Sugi; H.
    Biochimica et Biophysica Acta, May 2001, Refereed, Not invited
    Corresponding
  • 蛍光ATPアナログの筋収縮研究への応用
    茶圓 茂
    日本ME学会雑誌, 2001, Not refereed, Not invited
    Lead
  • Measurement of nucleotide exchange rate constants in single rabbit soleus myofibrils during shortening and lengthening using a fluorescent ATP analog.
    Shirakawa; I.; Chaen. S.; Bagshaw; C. R. and Sugi; H.
    Biophys. J., Sep. 2000, Refereed, Not invited
    Corresponding
  • Comparison of unitary displacements and forces between cardiac myosin isoforms by the optical trap technique: Molecular basis for cardiac adaptation.
    Sugiura; S.; Kobayakawa; N.; Fujita; H.; Yamashita; H.; Momomura; S.; Chaen; S.; Omata; M. and Sugi; H.
    Circulation Res., 1998, Refereed, Not invited
  • Evanescent excitation microscopy. Its application to the study of single molecular process kinetics of actomyosin motor.
    Miyata; H.; Chaen; S.; Sugiura; S. and Sugi; H.
    Adv. Exp. Med. Biol., 1998, Refereed, Not invited
  • ATP-induced axial movement of myosin heads in living thick filaments recorded with a gas environmental chamber attached to the electron microscopy.
    Sugi; H.; Akimoto; T.; Chaen; S. and Suzuki; S.
    Adv. Exp. Med. Biol., 1998, Refereed, Not invited
  • Carp expresses fast skeletal myosin isoforms with altered motor functions and structural stabilities to compensate for changes in environmental temperature.
    Watabe; S.; Hirayama; Y.; Nakaya; M.; Kakinuma; M.; Kikuchi; K.; Guo; X-F.; Kanoh; S.; Chaen; S.; and Ooi; T.
    J. Therm. Biol., 1998, Refereed, Not invited
  • Distinct kinetic properties of cardiac myosin isoforms revealed by in vitro studies.
    Sugiura; S.; Lobayakawa; N.; Fujita; H.; Momomura; S.; Chaen; S. and Sugi; H.
    Adv. Exp. Med. Biol., 1998, Refereed, Not invited
  • Measurement of ATP turnover during shortening and lengthening of rabbit psoas myofibrils using fluorescent ATP analog.
    Chaen; S.; Shirakawa; I.; Bagshaw; C. R. and Sugi; H.
    Adv. Exp. Med. Biol., 1998, Refereed, Not invited
    Lead
  • Measurement of nucleotide release kinetics in single skeletal muscle myofibril during isometric and isovelocity contractions using fluorescence microscopy.
    Chaen; S.; Shirakawa; I.; Bagshaw; C. R. and Sugi; H.
    Biophys. J., Jul. 1997, Refereed, Not invited
    Lead
  • Dynamic electron microscopy of ATP-induced myosin head movement in living muscle thick filaments.
    Sugi; H.; Akimoto; T.; Sutoh; K.; Chaen; S.; Oishi; N. and Suzuki; S.
    Proc. Natl. acad. Sci. USA, 1997, Refereed, Not invited
  • Lower activation energy for sliding of F-actin on a less thermostable isoform of carp myosin.
    Chaen; S.; Nakaya; M.; Guo; X-F.; and Watabe; S.
    J. Biochem., Jun. 1996, Refereed, Not invited
    Lead
  • Different cardiac myosin isoforms exhibit equal force-generating ability in vitro.
    Sugiura; S.; Kobayakawa; N.; Momomura; S.; Chaen; S.; Omata; M; and Sugi; H.
    B. B. A., 1996, Refereed, Not invited
  • Effect of lateral forces on the movement of myosin-coated beads on actin cables studied using a centrifuge microscope.
    Kobayashi; T.; Gross; T.; Chaen; S.; Sugiura; S.; Tanaka; H. and Sugi; H.
    Jpn. J. Physiol., 1995, Refereed, Not invited
  • The mode of ATP-dependent microtuble-kinesin sliding in the auxotonic condition.
    Shirakawa; I.; Oiwa; K.; Chaen; S.; Shimizu; T.; Tanaka; H. and Sugi; H.
    J. Exp. Biol., 1995, Refereed, Not invited
  • The force-velocity relationship of the ATP-dependent actin-myosin sliding causing cytoplasmic streaming in algal cells, studied using a centrifuge microscope.
    Chaen; S.; Inoue; J. and Sugi; H.
    J. Exp. Biol., 1995, Refereed, Not invited
    Lead
  • Force-velocity relations of rat cardiac myosin isozymes sliding on algal cells actin cables in vitro.
    Sugiura; S.; Yamashita; H.; Sata; M.; Momomura; S.; Serizawa; T.; Oiwa; K.; Chaen; S.; Shimmen; T.; and Sugi; H.
    B. B. A., 1995, Refereed, Not invited
  • Kinetic properties of the ATP-dependent actin-myosin sliding as revealed by the force-movement assay system with a centrifuge microscope.
    Chaen; S.; Oiwa; K.; Kobayashi; T.; Gross; T.; Kamitsubo; E.; Shimmen; T. and Sugi; H.
    Adv. Exp. Med. Biol., 1993, Refereed, Not invited
    Lead
  • Dependence of the work done by ATP-induced actin-myosin sliding on the initial baseline force: its implications for kinetic properties of myosin heads in muscle contraction.
    Sugi; H.; Oiwa; K. and Chaen; S.
    Adv. Exp. Med. Biol., 1993, Refereed, Not invited
  • Relation between magnetically-applied force and velocity in beads coated with rabbit myosin, sliding on actin cables in Nitellopsis cells.
    Tregear; R.; Oiwa; K.; Chaen; S. and Sugi; H.
    J. Muscle Res. Cell Motil., 1993, Refereed, Not invited
  • Measurement of work done by ATP-induced sliding between rabbit muscle nyosin and slgal cell actin cables in vitro.
    Oiwa; K.; Chaen; S. and Sugi; H.
    J. Physiol., 1991, Refereed, Not invited
  • Characterization of the translocator associated pollen tube organelles.
    Kohno; T.; Chaen; S. and Shimmen; T.
    Plotoplasma, 1990, Refereed, Not invited
  • Steady-state force-velocity relation in the ATP-dependent sliding movement of myosin-coated beads on actin cables in vitro studied with a centrifuge microscope.
    Oiwa; K.; Chaen; S.; Kamitsubo; E.; Shimmen; T. and Sugi; H.
    Proc. Natl. acad. Sci. USA, 1990, Refereed, Not invited
  • Simultaneous recordings of force and sliding movement between myosin-coated glass microneedle and actin cables in vitro.
    Chaen; S.; Oiwa; K.; Shimmen; T.; Iwamoto; H. and Sugi; H.
    Proc. Natl. acad. Sci. USA, 1989, Refereed, Not invited
    Lead
  • Cooperative interactions of myosin heads in muscle force generation.
    Chaen; S.; Shimada; M. and Sugi; H.
    Adv. Exp. Med. Biol., 1988, Refereed, Not invited
    Lead
  • Evidence for cooperative interaction of myoshin heads with thin filament in the force generation of vertebrate skeletal muscle fibers.
    Chaen; S.; Shimada; M. and Sugi; H.
    J. Biol. Chem., 1986, Refereed, Not invited
    Lead
  • Fluorescence properties and contraction characteristics of ANM (N-(1-anilinonaphty1-4)maleimide)-Labeled rabbit psoas muscle fibers.
    Chaen; S.; Shimada; M. and Sugi H.
    J. Biochem., 1985, Refereed, Not invited
    Lead
  • Fluorescence properties and contraction characteristics of ANM (N-(1-anilinonaphty1-4)maleimide)-Labeled rabbit psoas muscle fibers.
    Chaen, S; Shimada, M; Sugi H
    J. Biochem., 1985, Refereed, Not invited
  • Substrate-concentration dependence of contraction parameters in glycerinated insect flight muscle fibers from Lethocerus derollei.
    Chaen; S. and Shimizu; H.
    J. Biochem., 1984, Refereed, Not invited
    Lead
  • The mode of action of maitotoxin on the membrane systems of frog skeletal muscle fibers.
    Gomi; S.; Chaen; S. and Sugi; H.
    Proc. Japan Acad., 1984, Refereed, Not invited
  • Substrate concentration dependence of contraction characteristics in glycerinated horseshoe crab muscle fibers.
    Chaen; S. and Sugi; H.
    Comp. Biochem. Physiol., 1982, Refereed, Not invited
    Lead
  • Substrate-concentration dependence of tension, shortening velocity and ATPase activity of glycerinated single muscle fibers.
    Chaen; S.; Kometani; K.; Yamada; T. and Shimizu; H.
    J. Biochem., 1981, Refereed, Not invited
    Lead
  • Models of muscle contraction.
    Chaen; S.
    Teikyo Med. J., 1980, Refereed, Not invited
    Lead
  • Active potential and synamic cooperativity in the chemomechanical conversion in active streaming and muscle contraction.
    Shimizu; H.; Yano; M.; Nishiyama; K.; Kometani; K.; Chaen; S. and Yamada; T.
    In Cross-Bridge Mechanism in Muscle Contraction H. Sugi and G. H. Pollack, editors University of Tokyo Press, Tokyo, 1979, Refereed, Not invited
  • The three state model for the elementary process of energy conversion in muscle.
    Nishiyama; K.; Shimizu; H.; Kometani; K. and Chaen; s.
    Biochim. Biophys. Acta, 1977, Refereed, Not invited

MISC

  • Definite Differences between In Vitro Actin-Myosin Sliding and Muscle Contraction as Revealed Using Antibodies to Myosin Head.
    Sugi H; Chaen S; Kobayashi T; Abe T; Kimura K; Saeki Y; Ohnuki Y; Miyakawa T; Tanokura M; SUgiura S.
    PLoS One, 11 Jun. 2014, Refereed, Not invited

Lectures, oral presentations, etc.

  • ★Effects of trimethylamine-N-oxide on the thermal stability of myosin
    Shigeru Chaen
    The 59th Annual meeting of the Biophysical Society of Japan, Nov. 2021, 日本生物物理学会, Not invited
  • Single amino acid substitution for the fungal kinesin offers possible Cold-adaptation but impairs thermal stability
    Shimizu Y.; Togawa T.; Chaen S.
    第56回日本生物物理学会年会, Sep. 2018
  • Properties of kinesics from filamentous fungi focused on the thermal aptitude
    Shimizu; Y.; Togawa; T.; Chaen; S.
    The 55th Annual Meeting of the Biophysical Society of Japan, Sep. 2017, Not invited
  • 37℃至適の糸状菌キネシンの特性調査と、低温適性をもたらす残基の探索
    清水洋輔、外川徹、茶圓茂
    2017年生体運動研究合同班会議, Jan. 2017, Not invited
  • Myosin II SH1 helix mutant lowers the activation energy fo sliding of Factin
    Chaen; S.; Shibata; K.; Koyama; T.; Suenaga; S.; Iwai; S.
    The 54th Annual Meeting of the Biophysical Society of Japan, Nov. 2016, Not invited
  • Motor function analysis of SH1 helix mutated myosin II causing myopathy and MYH9 disorder
    Shibata; K.; Koyama; T.; Iwai; S.; Chaen; S.
    60th Biophysical society Annual Meeting, Mar. 2016, Not invited
  • Motile activity and thermal stability of SH1 helix mutant myosin II
    hibata; K.; Iwai; S.; Chaen; S.
    The 53nd Annual Meeting of the Biophysical Society of Japan, Sep. 2015, Not invited
  • Mutating the SH1 helix region of Dictyosterium myosin II impairs motile activities and thermal stability
    Shibata; K.; Koyama; T.; Iwai; S.; Chaen; S.
    The 59th Biophysical society Annual Meeting, Feb. 2015, Not invited
  • Mutations in SH1 helix affect the motile activity of Dictyostelium myosin II
    shibata; K.; Koyama; T.; Iwai; S.; Chaen; S.
    The 52nd Annual Meeting of the Biophysical Society of Japan, Sep. 2014, Not invited
  • ガラス基板上の固定されたペリレンジカルボン酸誘導体の単一分子レベル挙動評価
    持田智朗、大月穣、須川晃資、茶圓茂、東條正
    日本化学会第94回春季年会, Mar. 2014, Not invited
  • Nucleotide turnover rates of bipolar myosin filament during actin filament sliding
    Maruta; T.; Miyazaki; S.; Kobatake; T.; Chaen; S.
    The 51th Annual Meeting of the Biophysical Society of Japan, Oct. 2013, Not invited
  • Effect of mutation in the SH1 helix region of Dictyosterium myosin II on the motile characteristics
    Koyama; T.; Maruta; T.; Iwai; S.; Chaen; S.
    The 51th Annual Meeting of the Biophysical Society of Japan, Oct. 2013, Not invited
  • EFFECT OF MUTATION OF THE SH1 HELIX REGION OF DICTYOSTERIUM MYOSIN II ON THE MOTILE ACTIVITIES.
    Tsubasa Koyama; Takahiro Maruta; Sousuke Iwai; Shieru Chaen
    57th Biophysical society Annual Meeting, Feb. 2013, Not invited
  • MEASUREMENT OF NUCLEOTIDE RELEASE FROM SYNTHETIC MYOSIN THICK FILAMENT ALONG WHICH ACTIN FIAMENTS SLIDE
    Takahiro Maruta; Hiroyuki Okubo; Takahiro Kobateke; Shieru Chaen
    57th Biophysical society Annual Meeting, Feb. 2013, Not invited
  • Evidence that actin-myosin cycling in muscle may not pass through rigor configuration
    ugi; H.; Chaen; S.; Kobayashi; T.; Abe; T.
    57th Biophysical society Annual Meeting, Feb. 2013, Not invited
  • Effect of antibodies to myosin head revels definite difference between in vitro actin-myosin sliding and muscle contraction
    Abe; T.; Kobayashi; T.; Chaen; S.; Miyakawa; T.; Tanokura; S.; Ohnuki; Y.; Sugi; H.
    57th Biophysical society Annual Meeting, Feb. 2013, Not invited
  • Measurement of ADP release from bipolar myosin filament track Along which actin filament slide.
    Maruta; T.; Okubo; H.; Kobatake; T.m Chaen; S.
    The 50th Annual Meeting of the Biophysical Society of Japan, Sep. 2012, Not invited
  • Effect of mutation of the SH1 helix region of Dyctyosterium Myosin II on the motile characteristics.
    Koyama; T.; Maruta; T.; Iwai; S.; Chaen; S.
    The 50h Annual Meeting of the Biophysical Society of Japan, Sep. 2012, Not invited
  • Determination of the kinetic on- and off-rate between a plasma membrane and a lipid-anchored protein in a living cell by single molecule imaging technique
    東條 正、小畑貴広、秋山麻衣、井上智博、茶圓茂
    日本薬学会第132年会, Mar. 2012, Not invited
  • 生きている細胞の内部において脂肪酸鎖付加タンパク質が細胞膜に結合・解離する際の速度定数を蛍光1分子イメージングによって決定する
    東條正、小畑貴広、秋山麻衣、井上智博、茶圓茂
    日本物理学会第67回年次大会, Mar. 2012, Not invited
  • Definite difference between in vitro actin-myosin sliding and muscle contraction revealed by the effect of antibody to myosin head converter domain
    Haruo Sugi; Takakazu Kobayashi; Shigeru Chaen; Takuya Miyakawa; Masaru Tanokura; Yasutake Saeki; Hiroki Minoda
    56th Biophysical society Annual Meeting, Feb. 2012, Not invited
  • Development of methods to observe a living cell and macromolecules in aqueous solution with scanning electron microscope at nanometer resolution
    Tadashi Tojo; Naoki Ogawa; Akira Ishikawa; Shigeru Chaen
    56th Biophysical society Annual Meeting, Feb. 2012, Not invited
  • Observation of sliding movement of actin filament changing the myosin filament track
    Ohkubo; H.; Shimokawa; T.; Mogami; T.; Chaen; S.
    The 49th Annual Meeting of the Biophysical Society of Japan, Sep. 2011, Not invited
  • Mutating the SH1 helix region of Dictyosterium myosin II impairs actin-myosin motility
    Koyama; T.; Maruta; T.; Hukushima; Y.; Iwai; S.; Chaen; S.
    The 49th Annual Meeting of the Biophysical Society of Japan, Sep. 2011, Not invited
  • Thermal activation energy for bidirectional movement of actin filament along bipolar tracks of myosin filaments
    H. Okubo; M. Iwai; S. Iwai and S. Chaen
    The 48th Annual Meeting of the Biophysical Society Of Japan, Sep. 2010, Not invited
  • Temperature dependence of sliding velocity of actin filaments along bipolar tracks of myosin filament.
    H. Okubo; M. Iwai; T. Kobatake; S. Chaen
    The 47th Annual Meeting of the Biophysical Society Of Japan, Nov. 2009, Not invited
  • Kinesin calmodulin fusion protein as a molecular shuttle
    Shishido; H.; Nakazato; K.; Katayama; E.; Maruta; S.
    The 47th Annual Meeting of the Biophysical Society Of Japan, Oct. 2009, Not invited
  • Interaction of Processive Motor Proteins with ATP analogue Having Syn conformation with respect to the Adenine-ribose bond.
    Kazuhiro Kawanoue; Shigeru Chaen; Shinsaku Maruta
    Biophysical Society, Mar. 2009, Not invited
  • Energy barriers for bi-directional movement of F-actin along myosin thick filament
    Chaen; S.; Morioka; K.
    日本生物物理学会, Dec. 2008, Not invited
  • ミオシンのSH1へリックス領域の変異が弾性と熱安定性に及ぼす影響
    茶圓 茂/岩井草介
    生体合同班会議, Jan. 2007, Not invited
  • A point mutation in the SH1 helix alters elasticity and thermal stability of myosin II.
    岩井草介/花元大輔、茶圓 茂
    日本生物物理学会, Nov. 2006, Not invited
  • ミオシンのATP依存的な構造変化に含まれる拡散的な過程
    岩井草介/須藤和夫、茶圓 茂
    日本生物物理学会, 2005, Not invited
  • SH1へリックスに変異を導入したミオシンの運動機能解析
    花元大輔/岩井草介、須藤和夫、茶圓 茂
    日本生物物理学会, 2005, Not invited
  • 滑り運動活性化エネルギーの低いミオシンアイソフォーム
    茶圓 茂/岩井草介、須藤和夫、渡部終五
    日本生物物理学会, 2003, Not invited
  • In vitro actomyosin motility in deuterium oxide
    Chaen; S./ Yamamoto; N.; Shirakawa; I.; and Sugi; H.
    2002, Not invited
  • Effect of partial inactivation of myosin heads on the ATP-dependent actin myosin sliding
    Muraoka; A. and Sugi; H
    Biophysical society Annual Meeting, Feb. 2001, Not invited
  • 蛍光ATPを用いた短縮中筋原線維ATP分解キネティクスの研究
    茶圓 茂/白川伊吹、C.R. Bagshaw; 杉晴夫
    日本生理学会, 2001, Not invited
  • 重水中のアクチンミオシン滑り運動
    茶圓 茂/山本直人、白川伊吹、杉晴夫
    日本生物物理学会, 2001, Not invited
  • Interaction of fluorescent ribose-modified ATP analogue with skeletal muscle
    Maruta; S.; Mizukura; Y.; Aihara; T.
    44th Biophysical society Annual Meeting, Feb. 2000, Not invited
  • 蛍光性ATPを用いた短縮中、伸長中のウサギ遅筋の筋原線維内ヌクレオチド遊離速度の測定
    白川伊吹/茶圓 茂、杉晴夫
    日本生理学会, 2000, Not invited
  • 蛍光性ATPアナログのATPase siteへの結合時間のビデオ高速イメージング
    茶圓 茂/白川伊吹、原田慶恵
    日本生物物理学会, 2000, Not invited
  • 重水(D2O)環境下でのウサギ骨格筋ミオシン上のアクチン滑り速度
    茶圓 茂/山本直人、杉晴夫
    日本生物物理学会, 1999, Not invited
  • アクトミオシンATP反応のビデオ高速イメージング
    茶圓 茂/白川伊吹、杉晴夫
    日本生物物理学会, 1999, Not invited
  • Measurement of nucleotide release in contracting muscle myofibrils using fluorescent ATP analog.
    Shirakawa; I.; Bagshaw; C. R. and Sugi; H.
    International congress of physiological sciences, Jun. 1997, Not invited
  • ストレイン依存アクトミオシンATP分解サイクルの解析
    茶圓 茂/白川伊吹、C.R.Bagshaw; 杉晴夫
    日本生物物理学会, 1997, Not invited
  • 短縮中の骨格筋筋原線維内ADP遊離の直接測定
    茶圓 茂/白川伊吹、C.R.Bagshaw; 杉晴夫
    日本生物物理学会, 1996, Not invited
  • 蛍光性ATPアナログを用いた、短縮中のウサギ骨格筋筋原線維内ATP分解KINETICSの解析
    茶圓 茂/白川伊吹、C.R.Bagshaw; 杉晴夫
    日本生物物理学会, 1995, Not invited
  • ATPase activity and isometric force generated by single skeletal myofibers in hypertrophic cardiomyopathy associated with b-myosin heavy chain gene mutations.
    Horowits; R.; Podolsky; R. J.; Epstein; N. D. and Fananapazir; L.
    Biophysical society Annual Meeting, Feb. 1994, Not invited
  • 家族性肥大型心筋症患者骨格筋単一筋線維のATP分解活性と等尺性張力
    茶圓 茂/R. Horowits; R.Podolsky; N.D.Epstein; L.Fananapazir
    日本生物物理学会, 1994, Not invited
  • Dependence on the internal force of apparent efficiency of the ATP-induced actin-myosin sliding in producing work studied using an in vitro force movement assay system.
    Sugi; H.; Oiwa; K.
    Biophysical society Annual Meeting, Feb. 1991, Not invited
  • Relation between the amount of work done by actin-myosin sliding and amount of iontophoretically applied ATP studied using an in vitro assay system.
    Oiwa; K.; Sugi; H.
    Biophysical society Annual Meeting, Feb. 1991, Not invited
  • 化学修飾ミオシン(S1,HMM)上のアクチン線維の滑り
    茶圓 茂/村岡明子
    日本生物物理学会, 1990, Not invited
  • ATP iontophoresis によるアクチンーミオシン相互作用の解析
    大岩和弘/茶圓 茂、杉晴夫
    日本生物物理学会, 1990, Not invited
  • In vitro 実験系によるアクチンーミオシン間の滑り力と滑り速度の定常状態における関係
    杉晴夫/茶圓 茂、大岩和弘、上坪英治、新免輝男
    日本生物物理学会, 1990, Not invited
  • 遠心顕微鏡によるin vitro アクチンーミオシン系の滑り運動の解析
    小林孝和/茶圓 茂、大岩和弘、上坪英治、新免輝男、杉晴夫
    日本生物物理学会, 1990, Not invited
  • Basic properties of actin-myosin interaction in muscle as studied using a new in vitro assay system
    Oiwa; K.; Shimmen; T. and Sugi; H.
    International congress of physiological sciences, Jul. 1989, Not invited
  • 車軸藻アクチンケーブル上でのミオシン滑り運動の力学特性
    大岩和弘/茶圓 茂、新免輝男、杉晴夫
    日本生物物理学会, 1989, Not invited
  • アクチンーミオシン間の滑りと力発生の直接的力学測定
    茶圓 茂/大岩和弘、新免輝男、杉晴夫
    日本生物物理学会, 1989, Not invited
  • アクチンケーブル上のミオシン被覆ビーズの滑り運動
    茶圓 茂/新免輝男、杉晴夫
    日本生物物理学会, 1988, Not invited
  • 花粉管オルガネラの車軸藻アクチン繊維上における運動の解析
    河野匡/茶圓 茂、新免輝男
    日本生物物理学会, 1988, Not invited
  • 車軸藻アクチン線維束上のpPDM修飾ミオシンの動き
    茶圓 茂/新免輝男、杉晴夫
    日本生物物理学会, 1987, Not invited
  • p-PDM修飾筋線維の収縮特性
    茶圓 茂/島田光世、杉晴夫
    日本生物物理学会, 1985, Not invited
  • p-PDM処理筋線維の張力
    茶圓 茂/島田光世、杉晴夫
    日本生物物理学会, 1984, Not invited
  • 蛍光ラベルグリセリン処理ウサギ腸腰筋の硬直、弛緩、収縮状態に蛍光スペクトル
    茶圓 茂/杉晴夫
    日本生理学会, 1983, Not invited
  • ANMラベル筋線維の蛍光
    茶圓 茂/島田光世、杉晴夫
    日本生物物理学会, 1983, Not invited
  • カブトガニグリセリン筋収縮特性の基質濃度依存性
    茶圓 茂/杉晴夫
    日本生理学会, 1982, Not invited
  • 蛍光ラベル軽鎖交換ミオシンの蛍光
    茶圓 茂
    日本生物物理学会, 1982, Not invited
  • カブトガニグリセリン筋収縮特性の基質濃度依存性
    茶圓 茂/杉晴夫
    日本生物物理学会, 1981, Not invited
  • 骨格筋における等張性収縮現象シミュレーション
    茶圓 茂/米谷快男児、土屋禎三、杉晴夫
    日本生物物理学会, 1980, Not invited
  • グリセリン処理したタガメ飛翔筋の収縮特性の基質濃度依存性
    茶圓 茂/山田武範、清水博
    日本薬学会, 1980, Not invited
  • Computer simulation of isotonic shortening in frog skeletal muscle fibers
    杉晴夫/茶圓 茂、土屋禎三
    日本動物学会, 1980, Not invited
  • 種々の基質環境下におけるグリセリン処理家ウサギ腸腰筋の収縮特性
    茶圓 茂/米谷快男児、山田武範
    日本生物物理学会, 1979, Not invited

Affiliated academic society

  • Jan. 1990 - Present
    Biophysical Society U.S.A.
  • 日本生物物理学会
  • Japan Biophysical Society