KISHI Tsutomu
| Department of Chemical Biology and Applied Chemistry | Professor |
Researcher Information
Research activity information
Paper
- A Set of Plasmid-Based Modules for Easy Switching of C-Terminal Epitope Tags in Saccharomyces cerevisiae
Hiroki Hayashi; Tsutomu Kishi
Microorganisms, Dec. 2021, Refereed - ARID2 is a pomalidomide-dependent CRL4CRBN substrate in multiple myeloma cells.
Junichi Yamamoto; Tetsufumi Suwa; Yuki Murase; Shumpei Tateno; Hirotaka Mizutome; Tomoko Asatsuma-Okumura; Nobuyuki Shimizu; Tsutomu Kishi; Shuji Momose; Masahiro Kizaki; Takumi Ito; Yuki Yamaguchi; Hiroshi Handa
Nature chemical biology, Nov. 2020, Refereed - Marker-free insertion of a series of C-terminal epitopes based on the 50:50 method in <i>Saccharomyces cerevisiae</i>
Masamichi Nonaka; Tsutomu Kishi
The Journal of General and Applied Microbiology, 2018, Refereed - New plasmids for the disruption and repeated use of selection markers in <i>Saccharomyces cerevisiae</i>
Kenshiro Nihei; Tsutomu Kishi
The Journal of General and Applied Microbiology, 2017, Refereed - Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function.
Yuan Liu; Kunio Nakatsukasa; Michiko Kotera; Akira Kanada; Takashi Nishimura; Tsutomu Kishi; Satoru Mimura; Takumi Kamura
Molecular biology of the cell, May 2011, Refereed - Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Kanako Kume; Yosuke Iizumi; Masashi Shimada; Yuki Ito; Tsutomu Kishi; Yuki Yamaguchi; Hiroshi Handa
Genes to cells : devoted to molecular & cellular mechanisms, Apr. 2010, Refereed - SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast.
Satoru Mimura; Makiko Komata; Tsutomu Kishi; Katsuhiko Shirahige; Takumi Kamura
The EMBO journal, Dec. 2009, Refereed - A longevity protein, Lag2, interacts with SCF complex and regulates SCF function.
Yuan Liu; Satoru Mimura; Tsutomu Kishi; Takumi Kamura
The EMBO journal, Nov. 2009, Refereed - A refined two-hybrid system reveals that SCF(Cdc4)-dependent degradation of Swi5 contributes to the regulatory mechanism of S-phase entry.
Tsutomu Kishi; Akemi Ikeda; Noriko Koyama; Junji Fukada; Rina Nagao
Proceedings of the National Academy of Sciences of the United States of America, Sep. 2008, Refereed - Oxidative stress-induced ubiquitination of RCAN1 mediated by SCFbeta-TrCP ubiquitin ligase.
Sachie Asada; Akemi Ikeda; Rina Nagao; Hiroshi Hama; Tatsuhiko Sudo; Akiyoshi Fukamizu; Yoshitoshi Kasuya; Tsutomu Kishi
International journal of molecular medicine, Jul. 2008, Refereed - Mutation of DNA primase causes extensive apoptosis of retinal neurons through the activation of DNA damage checkpoint and tumor suppressor p53
Masahiro Yamaguchi; Noriko Fujimori-Tonou; Yukihiro Yoshimura; Tsutomu Kishi; Hitoshi Okamoto; Ichiro Masai
DEVELOPMENT, Apr. 2008, Refereed, Not invited - The SCFCdc4 ubiquitin ligase regulates calcineurin signaling through degradation of phosphorylated Rcn1, an inhibitor of calcineurin.
Tsutomu Kishi; Akemi Ikeda; Rina Nagao; Noriko Koyama
Proceedings of the National Academy of Sciences of the United States of America, Oct. 2007, Refereed - Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.
Hiroaki Seino; Tsutomu Kishi; Hideo Nishitani; Fumiaki Yamao
Molecular and cellular biology, May 2003, Refereed
MISC
- A Set of Plasmid-Based Modules for Easy Switching of C-Terminal Epitope Tags in Saccharomyces cerevisiae.
Hiroki Hayashi; Tsutomu Kishi
Microorganisms, 03 Dec. 2021 - Switching off the genetic circuit that suppresses CDK activity
Switching off; he; genetic circuit that suppresses CDK activity; T. Kishi
化学系学協会, Sep. 2021, Not refereed, Invited - ARID2 is a pomalidomide-dependent CRL4CRBN substrate in multiple myeloma cells.
Junichi Yamamoto; Tetsufumi Suwa; Yuki Murase; Shumpei Tateno; Hirotaka Mizutome; Tomoko Asatsuma-Okumura; Nobuyuki Shimizu; Tsutomu Kishi; Shuji Momose; Masahiro Kizaki; Takumi Ito; Yuki Yamaguchi; Hiroshi Handa
Nature chemical biology, Nov. 2020 - Marker-free insertion of a series of C-terminal epitopes based on the 50:50 method in Saccharomyces cerevisiae.
Masamichi Nonaka; Tsutomu Kishi
The Journal of general and applied microbiology, 21 May 2018 - New plasmids for the disruption and repeated use of selection markers in Saccharomyces cerevisiae.
Kenshiro Nihei; Tsutomu Kishi
The Journal of general and applied microbiology, 11 Jul. 2017 - SCFCdc4-dependent degradation of Swi5 is confined within a narrow window in G1 phase
H. Hayashi; T. Kishi
Genes Genet. Syst., Mar. 2015, Refereed - CDK is involved in the control of spindle checkpoint
N. Watanabe; H. Hayashi; S. Oku; T. Kishi
Genes Genet. Syst, Mar. 2015, Refereed - Non-SCF-type F-box protein Roy1/Ymr258c interacts with a Rab5-like GTPase Ypt52 and inhibits Ypt52 function.
Yuan Liu; Kunio Nakatsukasa; Michiko Kotera; Akira Kanada; Takashi Nishimura; Tsutomu Kishi; Satoru Mimura; Takumi Kamura
Molecular biology of the cell, May 2011 - Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway.
Kanako Kume; Yosuke Iizumi; Masashi Shimada; Yuki Ito; Tsutomu Kishi; Yuki Yamaguchi; Hiroshi Handa
Genes to cells : devoted to molecular & cellular mechanisms, 01 Apr. 2010 - SCFCdc4 regulates S phase entry through degradation of Sic1 and Swi5
岸 努
生体の科学, 15 Dec. 2009 - SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast.
Satoru Mimura; Makiko Komata; Tsutomu Kishi; Katsuhiko Shirahige; Takumi Kamura
The EMBO journal, 02 Dec. 2009 - A longevity protein, Lag2, interacts with SCF complex and regulates SCF function.
Yuan Liu; Satoru Mimura; Tsutomu Kishi; Takumi Kamura
The EMBO journal, 04 Nov. 2009 - A refined two-hybrid system reveals that SCF(Cdc4)-dependent degradation of Swi5 contributes to the regulatory mechanism of S-phase entry.
Tsutomu Kishi; Akemi Ikeda; Noriko Koyama; Junji Fukada; Rina Nagao
Proceedings of the National Academy of Sciences of the United States of America, 23 Sep. 2008 - Oxidative stress-induced ubiquitination of RCAN1 mediated by SCFbeta-TrCP ubiquitin ligase.
Sachie Asada; Akemi Ikeda; Rina Nagao; Hiroshi Hama; Tatsuhiko Sudo; Akiyoshi Fukamizu; Yoshitoshi Kasuya; Tsutomu Kishi
International journal of molecular medicine, Jul. 2008 - The SCFCdc4 ubiquitin ligase regulates calcineurin signaling through degradation of phosphorylated Rcn1, an inhibitor of calcineurin.
Tsutomu Kishi; Akemi Ikeda; Rina Nagao; Noriko Koyama
Proceedings of the National Academy of Sciences of the United States of America, 30 Oct. 2007 - Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.
Hiroaki Seino; Tsutomu Kishi; Hideo Nishitani; Fumiaki Yamao
Molecular and cellular biology, May 2003 - An essential function of Grr1 for the degradation of Cln2 is to act as a binding core that links Cln2 to Skp1
Tsutomu Kishi; Fumiaki Yamao
Journal of Cell Science, 18 Dec. 1998, Refereed
Lead - Selective and time-specific ubiquitination of G1 cyclin Cln2 by SCF Grr1.
岸努
日本遺伝学会大会プログラム・予稿集, 1998 - Grr1 functions in the ubiquitin pathway in Saccharomyces cerevisiae through association with Skp1
T. Kishi; T. Seno; F. Yamao
Molecular and General Genetics MGG, Jan. 1998, Refereed - Grr1 links G1 cyclin Cln2 with the ubiquitinated machinery.
岸努; 瀬野かん二; 山尾文明
日本遺伝学会大会プログラム・予稿集, 1997 - 出芽酵母CDC34の温度感受性株のサプレッサー遺伝子GRR1の解析
岸努; 瀬野かん二
日本分子生物学会年会プログラム・講演要旨集, 1996 - Effects of Altered Rgd (ARG-GLY-ASP) Sequences in the Primer Protein on the Protein Primed DNA Replication of Bacteriophage M2 in Vitro
Tsutomu Kishi; Kin-Ichiro Miura; Kouji Matsumoto; Hideo Hirokawa
The Journal of General and Applied Microbiology, 1994 - Complementation assay of primer protein: Gene expression systems of plasmid vectors support the infection of suppressor sensitive mutant phages M2 and .PHI.29.
Tsutomu KISHI; Kin-ichiro MIURA; Kouji MATSUMOTO; Hideo HIROKAWA
The Japanese Journal of Genetics, 1993, Refereed - Mechanism of;rotein;m;DNA replication of;B.subtilis phage M2
T Kishi; K Miura; K Matsumoto; H Hirokawa
Nucleic Acids Symp Ser, Dec. 1992, Refereed
Research Themes
- Regulation of Mphase progression by the SCFCdc4 ubiquitin ligase.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2011 - 2013
KISHI Tsutomu - The SCF ubiquitin ligase regulates calcineurin signaling through degradation of RCAN1, an inhibitor of calcineurin.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 2008 - 2011
KISHI Tsutomu - ユビキチン化によって活性が制御される蛋白質のスクリーニングと機能解析
日本学術振興会, 科学研究費助成事業, 2002 - 2004
岸 努 - SCFユビキチンリガーゼによる細胞機能制御と薬剤開発への応用
科学技術振興機構, 戦略的な研究開発の推進 戦略的創造研究推進事業 さきがけ, 2002 - 2003
岸 努 - SCFユビキチンリガーゼの標的蛋白質の網羅的スクリーニング
日本学術振興会, 科学研究費助成事業, 2002 - 2002
岸 努; 清野 浩明 - SCFユビキチンリガーゼの標的蛋白質の網羅的スクリーニング
日本学術振興会, 科学研究費助成事業, 2001 - 2001
岸 努; 清野 浩明 - ユビキチン系による染色体複製開始の制御
日本学術振興会, 科学研究費助成事業, 1998 - 1998
岸 努 - Cellular functions regulated by specific ubiquitin pathways
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, 1995 - 1997
YAMAO Fumiaki - G2-M期における核小体解離のユビキチン経路による制御
日本学術振興会, 科学研究費助成事業, 1995 - 1995
瀬野 悍二; 清野 浩明; 岸 努 - 核小体のG2-M期移行における代謝と動態のユビキチン系による制御
日本学術振興会, 科学研究費助成事業, 1995 - 1995
瀬野 悍二; 清野 浩明; 岸 努 - ユビキチン系の制御を受けるDNA修復と誘導突然変異機構
日本学術振興会, 科学研究費助成事業, 1995 - 1995
瀬野 悍二; 清野 浩明; 岸 努 - ユビキチン系酵素変異株を用いた哺乳類細胞の誘導性修復能の研究
日本学術振興会, 科学研究費助成事業, 1994 - 1994
瀬野 悍二; 岸 努
